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用电镜观察体外培养的淋巴细胞和胸腺细胞在用半胱氨酸孵育后细胞膜表面被膜的变化。

Changes in the cellular membrane surface coat of lymphocytes and thymocytes after incubation in vitro with cystein as revealed with electronmicroscopy.

作者信息

Borowicz J, Olszewska K, Roszkowski-Sliz W, Ryzewski J

出版信息

Folia Histochem Cytochem (Krakow). 1977;15(4):299-303.

PMID:304432
Abstract

Changes in the cellular membrane surface coat of lymphocytes and thymocytes after incubation with cystein in vitro were revealed with electronmicroscope, while performing the reaction with Ruthenium Red and Concanavaline A. Lymphocytes and thymocytes not incubated with cystein to which reaction with Ruthenium red and Cocanavaline A was applied have shown a well developed and preserved surface coat of the cellular membrane. Contrary to this finding when lymphocytes and thymocytes were incubated with cystein and thereafter treated with Ruthenium Red and Concanavaline A no reaction product on the surface of the cellular membrane was observed. The experimental results could indicate on the influence of cystein on the glycoside bonds.

摘要

在用半胱氨酸体外孵育淋巴细胞和胸腺细胞后,通过电子显微镜观察了细胞膜表面被覆物的变化,同时进行了钌红和刀豆球蛋白A反应。未用半胱氨酸孵育但进行了钌红和刀豆球蛋白A反应的淋巴细胞和胸腺细胞,其细胞膜表面被覆物发育良好且保存完好。与此发现相反,当淋巴细胞和胸腺细胞用半胱氨酸孵育,然后用钌红和刀豆球蛋白A处理时,在细胞膜表面未观察到反应产物。实验结果可能表明半胱氨酸对糖苷键有影响。

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