Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
College of Life Sciences, Zhejiang Sci-Tech University, Road 2, Hangzhou, 310018, Zhejiang, China.
Curr Microbiol. 2019 Sep;76(9):1082-1086. doi: 10.1007/s00284-018-1597-7. Epub 2018 Nov 15.
Massilia oculi sp. nov. of type strain CCUG 43427 is a Gram-negative, rod-shaped, nonspore-forming bacterium, which was recently isolated from the eye of a patient suffering from endophthalmitis and was described as novel species in Massilia genus. In this study, we present the complete genome sequence of this strain by using Pacbio SMRT cell platform and compare this sequence with the genomes of 30 Massilia representative strains. Also, a comprehensive search was conducted for genes and proteins involved in antibiotic resistance and pathogenicity. The genome of CCUG 43427 is 5,844,653 bp with 65.55% GC content. This genome contains four prophages and four genomic islands (GIs). The cobalt/zinc/cadmium transporter locus CzcABCD is included in these GIs. This GI was predicted to play important role in bacterial heavy-metal tolerance. The in silico genome analysis also revealed that this strain contains a lot of antibiotic resistance and pathogenicity related genes. This result suggested that this strain may has evolved a wide arsenal of weapons for pathogenicity and survival. Genome comparison among CCUG 43427 and other 30 Massilia strains revealed that more than 400 genes are unique in CCUG 43427. Among these, one gene cluster, which was annotated to be important for LOS biosynthesis, catalytic mechanism and the substrate specificity of the enzyme, was predicted to be horizontally transferred by using phylogenies and biased GC content.
新型马赛眼球菌（Massilia oculi sp. nov.）CCUG 43427 株为革兰氏阴性、杆状、非孢子形成菌，最近从患有眼内炎的患者眼部分离出来，并被描述为马赛菌属的新型物种。在本研究中，我们使用 Pacbio SMRT 细胞平台呈现了该菌株的完整基因组序列，并将该序列与 30 株马赛菌代表菌株的基因组进行了比较。此外，我们还进行了全面搜索，以寻找与抗生素耐药性和致病性相关的基因和蛋白质。CCUG 43427 的基因组为 5844653bp，GC 含量为 65.55%。该基因组包含四个噬菌体和四个基因组岛（GIs）。CzcABCD 钴/锌/镉转运体基因座包含在这些 GIs 中。预测该 GI 在细菌耐重金属方面发挥重要作用。通过计算机基因组分析还发现，该菌株含有大量与抗生素耐药性和致病性相关的基因。这一结果表明，该菌株可能已经进化出了广泛的致病和生存武器。CCUG 43427 与其他 30 株马赛菌的基因组比较表明，CCUG 43427 中存在 400 多个独特基因。其中，一个基因簇被注释为与 LOS 生物合成、催化机制和酶的底物特异性有关，通过系统发育和偏 GC 含量预测其发生了水平转移。
