年龄相关的大鼠肝脏中 GSH S-转移酶基因/蛋白质的变化。
Age-associated changes in GSH S-transferase gene/proteins in livers of rats.
机构信息
a Key Lab for Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine , Zunyi Medical University , Zunyi , People's Republic of China.
b Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica , Shanghai University of Traditional Chinese Medicine , Shanghai , People's Republic of China.
出版信息
Redox Rep. 2018 Dec;23(1):213-218. doi: 10.1080/13510002.2018.1546985.
OBJECTIVES
Glutathione S-transferases (GSTs) are phase-II metabolic enzymes playing important roles in drug metabolism, anti-oxidative stress and anti-aging. Age is a key factor influencing GSTs expression. Thus, age-related changes of 10 GSTs were examined.
METHODS
Livers from male Sprague-Dawley rats at fetus (-2 d), neonates (1, 7, 14 and 21 d), puberty (28 and 35 d), adulthood (60 and 180 d), and aging (540 and 800 d), were collected and subjected to qPCR analysis. Liver proteins from 14, 28, 60, 180, 540 and 800 d were also extracted for selected protein analysis by Western-blot.
RESULTS
The expression of GSTA1 and GSTP1 increased over the life span and the expression of GSTA4, GSTO1 and GSTZ1 gradually increased until adulthood, and slightly decreased at 800 days. The expression of GSTM1, GSTM3, GSTT1, GSTT2 and GSTK1 gradually increased until adulthood, but significantly decreased during aging of 540 and 800 days. There is a small peak at 7-14 d for GSTA1, GSTP1 and GSTZ1. The protein expression of GSTA1, GSTM1 and GSTP1 followed the trend of mRNA changes.
DISCUSSION
This study characterized three expression patterns of 10 GSTs during development and aging in rat liver, adding to our understanding of anti-aging role of GSTs.
目的
谷胱甘肽 S-转移酶（GSTs）是Ⅱ相代谢酶，在药物代谢、抗氧化应激和抗衰老中发挥重要作用。年龄是影响 GSTs 表达的关键因素。因此，本研究检测了 10 种 GSTs 的年龄相关性变化。
方法
收集雄性 Sprague-Dawley 大鼠胎期（-2 d）、新生期（1、7、14 和 21 d）、青春期（28 和 35 d）、成年期（60 和 180 d）和老年期（540 和 800 d）的肝脏，进行 qPCR 分析。还提取了 14、28、60、180、540 和 800 d 肝脏蛋白，通过 Western-blot 进行了选定蛋白的分析。
结果
GSTA1 和 GSTP1 的表达在整个生命过程中增加，GSTA4、GSTO1 和 GSTZ1 的表达逐渐增加到成年期，然后在 800 天略有下降。GSTM1、GSTM3、GSTT1、GSTT2 和 GSTK1 的表达逐渐增加到成年期，但在 540 和 800 天的衰老过程中显著下降。GSTA1、GSTP1 和 GSTZ1 在 7-14 d 时有一个小高峰。GSTA1、GSTM1 和 GSTP1 的蛋白表达与 mRNA 变化趋势一致。
讨论
本研究描述了 GSTs 在大鼠肝脏发育和衰老过程中的三种表达模式，增加了我们对 GSTs 抗衰老作用的理解。
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