Khamis Marwa Maher, Moselhy Said Salama, Rihan Shaimaa
Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Sci Rep. 2025 Mar 6;15(1):7879. doi: 10.1038/s41598-025-91027-7.
We evaluated the effect of trans-resveratrol (RSV) in ameliorating biochemical and molecular alterations in obese Wister male rats fed on high-fat/high-fructose-fed. Male Wister rats were divided into eight groups and fed with either a standard diet (control), high fructose (HF), high fat (HFAT), or a high- fructose high- fat (HF/HFAT) diet and supplemented with RSV (30 mg/kg/day) for 4 weeks. The food intake, body weight, glycemic parameters, lipid profile, oxidative stress were assessed. SIRT1 gene expression, PGC-1α, cyto-c and GLUT-4 were evaluated by qRT-PCR in adipose tissue of normal and obese rats. The body weight gain, serum fasting glucose, insulin, and HOMA-IR values were significantly higher in the HF and HF/HFAT groups than in the HFAT and control groups. Hyperlipidemia was observed in high calorie diets fed rats compared to control group. The levels of total cholesterol, triglycerides and LDL-c were significantly elevated while HDL- c was significantly decreased in HF & HF/HFAT groups compared to HFAT group. The levels of serum malondialdhyde (MDA) and superoxide dismutase (SOD) activity in adipose tissue were elevated in all groups compared to control group, particularly in the groups that were kept on a high fructose diets (HF, HF/HFAT). SIRT-1, PGC-1α, Cyto-c, and GLUT-4 genes levels were significantly down regulated in HF, HFAT & HF/HFAT groups compared to control group. Supplementation of T-RSV restored the alteration in carbohydrates-lipid metabolism as well as oxidative stress and upregulation of SIRT-1, PGC-1α, Cyto-c, and GLUT-4 genes. RSV is a promising treatment in the management of pathologic consequences of obesity from high-calorie diet consumption via molecular alteration of target genes.
我们评估了反式白藜芦醇（RSV）对高脂/高果糖喂养的肥胖雄性Wistar大鼠生化和分子改变的改善作用。雄性Wistar大鼠分为八组，分别喂食标准饮食（对照组）、高果糖（HF）、高脂肪（HFAT）或高果糖高脂肪（HF/HFAT）饮食，并补充RSV（30毫克/千克/天），持续4周。评估了食物摄入量、体重、血糖参数、血脂谱和氧化应激。通过qRT-PCR评估正常和肥胖大鼠脂肪组织中SIRT1基因表达、PGC-1α、细胞色素c和GLUT-4。HF组和HF/HFAT组的体重增加、血清空腹血糖、胰岛素和HOMA-IR值显著高于HFAT组和对照组。与对照组相比，高热量饮食喂养的大鼠出现高脂血症。与HFAT组相比，HF组和HF/HFAT组的总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平显著升高，而高密度脂蛋白胆固醇水平显著降低。与对照组相比，所有组脂肪组织中的血清丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性均升高，尤其是高果糖饮食组（HF、HF/HFAT）。与对照组相比，HF组、HFAT组和HF/HFAT组中SIRT-1、PGC-1α、细胞色素c和GLUT-4基因水平显著下调。补充反式白藜芦醇可恢复碳水化合物-脂质代谢以及氧化应激的改变，并上调SIRT-1、PGC-1α、细胞色素c和GLUT-4基因。RSV有望通过对靶基因的分子改变来治疗高热量饮食导致的肥胖病理后果。
