Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110 007, India.
Department of Biotechnology, Delhi Technological University, Bawana Road, New Delhi 110042, India.
Photochem Photobiol Sci. 2019 Jan 1;18(1):224-231. doi: 10.1039/c8pp00481a. Epub 2018 Nov 16.
The development of UV-B protective mechanisms in aquacultural species is essential for the sustainable production of healthy aqua crop. Freshwater carp Catla catla larvae (13.5 ± 1.12 mg) were fed with a diet containing 0.5% vitamin C (D1) and a control diet (D2) for 40 days. Each group was exposed to two doses of UV-B irradiation: 360 (5 min, D1 and D2) and 720 mJ cm (10 min, D1 and D2) for 15 days. Significantly (p < 0.05) higher survival and average weight were recorded in D1 compared to D2 exposed to the same dose. Also, significantly (p < 0.001) higher nitric oxide synthase and lower thiobarbituric acid reactive substances and heat shock protein 70 levels were recorded in D1 compared to the other groups. A direct relationship was found between the dose of UV-B and DNA fragmentation in muscles. DNA damage indices such as tail DNA, tail extent moment and olive tail moment were significantly (p < 0.01) lower in D1. Thus, supplementation of vitamin C in the diet provides UV-B protection to larvae.
水产养殖物种的 UV-B 保护机制的发展对于健康水产养殖的可持续生产至关重要。将含有 0.5%维生素 C（D1）的饲料和对照饲料（D2）分别投喂给 13.5±1.12mg 的淡水鲤鱼 Catla catla 幼鱼 40 天。每组幼鱼分别暴露于两种剂量的 UV-B 辐射下：360mJ/cm²（5min，D1 和 D2）和 720mJ/cm²（10min，D1 和 D2），共 15 天。与接受相同剂量照射的 D2 组相比，D1 组的存活率和平均体重显著更高（p<0.05）。此外，与其他组相比，D1 组的一氧化氮合酶水平显著更高（p<0.001），而丙二醛含量和热休克蛋白 70 水平显著更低。在肌肉中发现了 UV-B 剂量与 DNA 片段化之间的直接关系。D1 组的 DNA 损伤指数如尾部 DNA、尾部扩展矩和橄榄尾矩均显著更低（p<0.01）。因此，在饲料中添加维生素 C 可为幼鱼提供 UV-B 保护。
