Eliash N, Thangarajan S, Goldenberg I, Sela N, Kupervaser M, Barlev J, Altman Y, Knyazer A, Kamer Y, Zaidman I, Rafaeli A, Soroker V
Institute of Plant Protection, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel.
Institute of Agroecology and Plant Health, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
Insect Mol Biol. 2019 Jun;28(3):321-341. doi: 10.1111/imb.12553. Epub 2019 Jan 1.
The tight synchronization between the life cycle of the obligatory parasitic mite Varroa destructor (Varroa) and its host, the honeybee, is mediated by honeybee chemical stimuli. These stimuli are mainly perceived by a pit organ located on the distal part of the mite's foreleg. In the present study, we searched for Varroa chemosensory molecular components by comparing transcriptomic and proteomic profiles between forelegs from different physiological stages, and rear legs. In general, a comparative transcriptomic analysis showed a clear separation of the expression profiles between the rear legs and the three groups of forelegs (phoretic, reproductive and tray-collected mites). Most of the differentially expressed transcripts and proteins in the mite's foreleg were previously uncharacterized. Using a conserved domain approach, we identified 45 transcripts with known chemosensory domains belonging to seven chemosensory protein families, of which 14 were significantly upregulated in the mite's forelegs when compared to rear legs. These are soluble and membrane bound proteins, including the somewhat ignored receptors of degenerin/epithelial Na+ channels and transient receptor potentials. Phylogenetic clustering and expression profiles of the putative chemosensory proteins suggest their role in chemosensation and shed light on the evolution of these proteins in Chelicerata.
专性寄生螨类狄斯瓦螨（Varroa destructor，简称狄斯瓦螨）与其宿主蜜蜂之间的紧密同步是由蜜蜂的化学刺激介导的。这些刺激主要由位于螨前腿远端的一个凹陷器官感知。在本研究中，我们通过比较不同生理阶段螨的前腿与后腿之间的转录组和蛋白质组图谱，来寻找狄斯瓦螨的化学感应分子成分。总体而言，比较转录组分析显示后腿与三组前腿（携带型、繁殖型和从蜂箱中采集的螨）的表达谱有明显分离。螨前腿中大多数差异表达的转录本和蛋白质以前都未被表征。使用保守结构域方法，我们鉴定出45个具有已知化学感应结构域的转录本，它们属于七个化学感应蛋白家族，其中与后腿相比，有14个在螨的前腿中显著上调。这些是可溶性和膜结合蛋白，包括一些被忽视的退化蛋白/上皮钠离子通道受体和瞬时受体电位。推定的化学感应蛋白的系统发育聚类和表达谱表明它们在化学感应中的作用，并揭示了这些蛋白在螯肢动物中的进化情况。
