Diamond I, Legg A, Schneider J A, Rozengurt E
J Biol Chem. 1978 Feb 10;253(3):866-71.
Addition of serum to quiescent cultures of 3T3 cells rapidly increases lactic acid formation and subsequently stimulates cell division. The stimulation of lactic acid production is seen at high, saturating concentrations of extra-cellular glucose. It is dependent on the time of exposure and on the dose of serum and is not blocked by the addition of cycloheximide, puromycin, or actinomycin D. In contrast, serum only marginally affects glycolysis by rapidly growing 3T6 or SV40-3T3 cells. In addition to serum, epidermal growth factor (0.1 to 10 ng/ml) and insulin (10 to 500 ng/ml) cause a striking stimulation of glycolysis in quiescent 3T3 cells. Neither exogenous cyclic nucleotides nor ouabain effect the glycolytic response, but the presence of Ca2+ markedly influences the activation of glycolysis by epidermal growth factor and by insulin. A novel finding in this study is that homogenates prepared from quiescent cells treated with serum, epidermal growth factor, or insulin show increased glycolysis as compared with homogenates from nonstimulated cultures. This finding will allow further experimental analysis of the cause of increased glycolysis in rapidly proliferating cells.
向3T3细胞的静止培养物中添加血清会迅速增加乳酸生成，随后刺激细胞分裂。在细胞外葡萄糖浓度较高且达到饱和时，可观察到乳酸生成的刺激作用。它取决于暴露时间和血清剂量，并且不会被添加放线菌酮、嘌呤霉素或放线菌素D所阻断。相比之下，血清对快速生长的3T6或SV40 - 3T3细胞的糖酵解影响很小。除血清外，表皮生长因子（0.1至10 ng/ml）和胰岛素（10至500 ng/ml）会显著刺激静止3T3细胞中的糖酵解。外源性环核苷酸和哇巴因均不影响糖酵解反应，但Ca2+的存在会显著影响表皮生长因子和胰岛素对糖酵解的激活作用。本研究中的一个新发现是，与未受刺激培养物的匀浆相比，用血清、表皮生长因子或胰岛素处理的静止细胞制备的匀浆显示出糖酵解增加。这一发现将有助于对快速增殖细胞中糖酵解增加的原因进行进一步的实验分析。
