Tomasiak A, Tomasiak M, Bańkowski E
Haematologia (Budap). 1982;15(3):303-10.
The addition of bovine blood platelet homogenate to confluent cultures of three established mouse cell lines (L-929, Balb c/3T3 and SC) brings about a rapid increase of lactic acid formation. Homogenates prepared from quiescent cells previously treated with platelet breakdown products show an increased rate of glycolysis as compared with homogenates of nonstimulated cell cultures. Activities of hexokinase, pyruvate kinase and lactate dehydrogenase remained unaltered but the activity of phosphofructokinase increased by about 60 per cent. Transport of 2-deoxy-D-glucose into the cells was slightly stimulated. It is concluded that the transport of glucose was not a limiting factor in confluent 3T3 cells. The findings suggest that the increase of phosphofructokinase activity may have accounted for the changes in glycolysis seen in intact cells and cell-free homogenates prepared from cultures exposed to some unknown factor(s) present in platelet homogenate.
向三种已建立的小鼠细胞系（L-929、Balb c/3T3和SC）的汇合培养物中添加牛血小板匀浆会导致乳酸生成迅速增加。与未受刺激的细胞培养物匀浆相比，由先前用血小板裂解产物处理的静止细胞制备的匀浆显示糖酵解速率增加。己糖激酶、丙酮酸激酶和乳酸脱氢酶的活性保持不变，但磷酸果糖激酶的活性增加了约60%。2-脱氧-D-葡萄糖进入细胞的转运受到轻微刺激。得出的结论是，葡萄糖转运不是汇合的3T3细胞中的限制因素。这些发现表明，磷酸果糖激酶活性的增加可能是完整细胞以及从暴露于血小板匀浆中某些未知因子的培养物制备的无细胞匀浆中所见糖酵解变化的原因。
