Molecular Systems Engineering Lab, Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
Molecular Systems Engineering Lab, Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
Int J Biol Macromol. 2019 Feb 15;123:350-362. doi: 10.1016/j.ijbiomac.2018.11.100. Epub 2018 Nov 13.
Methanothermobacter thermautotrophicus ΔH (MTH) is a potential methanogen known to reduce CO with H for producing methane biofuel in thermophilic digesters. The genome of this organism contains ~50.5% conserved hypothetical proteins (HPs; operome) whose function is still not determined precisely. Here, we employed a combined bioinformatics approach to annotate a precise function to HPs and categorize them as enzymes, binding proteins, and transport proteins. Results of our study show that 315 (35.6%) HPs have exhibited well-defined functions contributing imperative roles in diverse cellular metabolism. Some of them are responsible for stress-response mechanisms and cell cycle, membrane transport, and regulatory processes. The genome-neighborhood analysis found five important gene clusters (dsr, ehb, kaiC, cmr, and gas) involving in the energetic metabolism and defense systems. MTH operome contains 223 enzymes with 15 metabolic subsystems, 15 cell cycle proteins, 17 transcriptional regulators and 33 binding proteins. Functional annotation of its operome is thus more fundamental to a profound understanding of the molecular and cellular machinery at systems-level.
产甲烷热原体ΔH（MTH）是一种已知能够利用 H 将 CO 还原为甲烷生物燃料的潜在产甲烷菌，存在于嗜热消化器中。该生物体的基因组包含约 50.5%的保守假定蛋白（HPs；操纵子），其功能尚未被准确确定。在这里，我们采用了一种组合的生物信息学方法对 HPs 进行了精确的功能注释，并将它们归类为酶、结合蛋白和转运蛋白。我们的研究结果表明，315 个（35.6%）HPs 具有明确的功能，对不同的细胞代谢过程起着至关重要的作用。其中一些蛋白与应激反应机制、细胞周期、膜转运和调控过程有关。基因组邻居分析发现了五个重要的基因簇（dsr、ehb、kaiC、cmr 和 gas），涉及能量代谢和防御系统。MTH 操纵子包含 223 种酶，涉及 15 种代谢子系统、15 种细胞周期蛋白、17 种转录调节因子和 33 种结合蛋白。因此，对其操纵子的功能注释对于在系统水平上深入理解分子和细胞机制更为基础。
