Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Am J Trop Med Hyg. 2013 Sep;89(3):518-9. doi: 10.4269/ajtmh.13-0162. Epub 2013 Jul 15.
Q fever is a widespread zoonosis caused by Coxiella burnetii, an obligate intracellular gram-negative bacterium. The investigation of C. burnetii infection in Zambian livestock was carried out using molecular detection techniques. A total of 489 cattle and 53 goat blood samples were collected from Chama, Chongwe, Monze, and Petauke districts in Zambia. Molecular screening by polymerase chain reaction was performed using C. burnetii-species-specific primers. In total, 38 cattle and 4 goat samples were positive. The prevalence of C. burnetii differed among the four sites, with Chama (Eastern province) recording the highest, although Monze (Southern province) did not record any case of the bacteria. This study reports the first genetic detection of C. burnetii in Zambia.
Q 热是一种由柯克斯体引起的广泛动物传染病,柯克斯体是一种专性细胞内革兰氏阴性细菌。本研究采用分子检测技术对赞比亚牲畜中的柯克斯体感染进行了调查。从赞比亚的 Chama、Chongwe、Monze 和 Petauke 地区采集了 489 份牛和 53 份山羊血液样本。使用柯克斯体种特异性引物进行聚合酶链反应的分子筛选。共有 38 份牛和 4 份山羊样本呈阳性。四种地点的柯克斯体流行率不同,其中 Chama(东部省)的流行率最高,尽管 Monze(南部省)没有记录到任何细菌病例。本研究报告了赞比亚首次对柯克斯体的基因检测。