Mohanta Uday Kumar, Watanabe Takuya, Ohari Yuma, Itagaki Tadashi
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan; Department of Pathogenic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan.
Parasitol Int. 2019 Apr;69:1-7. doi: 10.1016/j.parint.2018.11.002. Epub 2018 Nov 13.
Precise discrimination of Echinostoma species within the 'revolutum' group is quite difficult because of their morphological similarities. The objective of this study was to precisely characterize the echinostomes of ducks from Bangladesh based on both morphological and molecular characteristics. Two Echinostoma species were identified: E. revolutum and E. robustum. In the phylogenetic trees (ITS2 and nad1), E. revolutum and E. robustum belonged to their respective Eurasian clade, which is distinct from the American clade. These results suggest that both species have two distinct and geographically separated lineages, Eurasian and American. Our molecular and morphological data combined with previously published data supports the synonymy of E. robustum, E. miyagawai, and E. friedi previously based on either molecular or morphological evidence. This study thus improves our understanding of species diversity of the 'revolutum' group, particularly in Asia.
由于“卷棘口吸虫”组内棘口吸虫种类在形态上相似，因此精确鉴别它们颇具难度。本研究的目的是基于形态学和分子特征，精确鉴定来自孟加拉国鸭体内的棘口吸虫。鉴定出了两种棘口吸虫：卷棘口吸虫和粗壮棘口吸虫。在系统发育树（ITS2和nad1）中，卷棘口吸虫和粗壮棘口吸虫属于各自的欧亚分支，这与美洲分支不同。这些结果表明，这两个物种都有两个不同的、地理上分隔的谱系，即欧亚谱系和美洲谱系。我们的分子和形态学数据与先前发表的数据相结合，支持了之前基于分子或形态学证据将粗壮棘口吸虫、宫川棘口吸虫和弗里德棘口吸虫视为同物异名的观点。因此，本研究增进了我们对“卷棘口吸虫”组物种多样性的理解，尤其是在亚洲地区。
