Gu S L, Luo M Y, Guo W, Pan Y, Zhan X D
Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, China.
Co-first author.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Apr 7;32(2):191-194. doi: 10.16250/j.32.1374.2019271.
To investigate the morphological characteristics of in domestic ducks in Wuhu area, and to explore the feasibility of the cytochrome oxidase subunit-1 () gene as a molecular marker for the identification of .
was isolated from free-ranged domestic ducks in Wuhu area, and the parasites were stained and identified. In addition, the mitochondrial gene of was amplified using a PCR assay, and the amplification product was sequenced and aligned with the GenBank database to yield the homology for the identification of parasite species in combination with morphological findings. Intra-species comparison was done based on the gene sequence.
The prevalence of infection was 16.67% in domestic ducks in Wuhu area, and the adult was 6.6 to 13.2 mm in length. The size of the gene was approximately 660 bp, which had a 99.68% homology to the accessed in GenBank. The morphological findings were in agreement with molecular identification.
infection is common in domestic ducks in Wuhu area, and the mitochondrial gene is a feasible marker of intra- and inter-species molecular identification of .
调查芜湖地区家鸭体内[寄生虫名称未给出]的形态学特征，并探讨细胞色素氧化酶亚基-1（[基因名称未给出]）基因作为[寄生虫名称未给出]鉴定分子标记的可行性。
从芜湖地区放养的家鸭体内分离出[寄生虫名称未给出]，对寄生虫进行染色和鉴定。此外，采用聚合酶链反应（PCR）法扩增[寄生虫名称未给出]的线粒体[基因名称未给出]基因，对扩增产物进行测序，并与GenBank数据库进行比对，结合形态学结果得出同源性以鉴定寄生虫种类。基于[基因名称未给出]基因序列进行种内比较。
芜湖地区家鸭[寄生虫名称未给出]感染率为16.67%，成虫体长为6.6至13.2毫米。[基因名称未给出]基因大小约为660 bp，与GenBank中登录的[基因名称未给出]具有99.68%的同源性。形态学结果与分子鉴定结果一致。
[寄生虫名称未给出]感染在芜湖地区家鸭中较为常见，线粒体[基因名称未给出]基因是[寄生虫名称未给出]种内和种间分子鉴定的可行标记。
