Hoffmann C C, Moore K C, Shih C Y, Blakley R L
J Cell Sci. 1977 Dec;28:151-65. doi: 10.1242/jcs.28.1.151.
Preparations of human peripheral blood lymphocytes containing 72-87% T-cells and 10-16% B-cells were shown by scanning electron microscopy to consist almost exclusively of cells bearing numerous microvilli, whereas thymocytes were of mixed surface morphology, with both smooth and encrusted forms numerous. T-lymphocytes purified on long nylon columns were all covered with numerous short villi. Stimulation with phytohaemagglutinin for 2 days produced T-lymphoblasts almost exclusively, and as the T-cells enlarged the microvilli lengthened, the increase in length reaching 5-fold by day 3. Addition of sufficient methotrexate on day 3 to arrest proliferation (50 nM) caused progressive loss of microvilli from the cell surface, with the eventual production of large numbers of smooth cells, the surfaces of which later became pitted, followed by the complete dissolution of the cell. T-lymphocytes were shown to form rosettes with sheep erythrocytes through direct contact of the cell membranes over a significant area, but when, as a result of methotrexate treatment, the lymphocytes had become denuded of microvilli or had reached an advanced state of dissolution, rosettes were no longer formed.
通过扫描电子显微镜观察发现，含72 - 87% T细胞和10 - 16% B细胞的人外周血淋巴细胞制剂几乎完全由带有大量微绒毛的细胞组成，而胸腺细胞表面形态则较为多样，既有光滑的形态，也有大量带壳的形态。在长尼龙柱上纯化的T淋巴细胞全部覆盖着大量短绒毛。用植物血凝素刺激2天几乎只产生T淋巴母细胞，随着T细胞增大，微绒毛变长，到第3天长度增加达5倍。在第3天加入足够的甲氨蝶呤以阻止增殖（50 nM）会导致细胞表面微绒毛逐渐丧失，最终产生大量光滑细胞，其表面随后出现凹陷，接着细胞完全溶解。T淋巴细胞通过细胞膜在相当大的区域直接接触与绵羊红细胞形成玫瑰花结，但由于甲氨蝶呤处理，当淋巴细胞微绒毛脱落或达到晚期溶解状态时，就不再形成玫瑰花结。
