Kelly G E, Nockolds C E
Scand J Haematol. 1977 Aug;19(2):172-84. doi: 10.1111/j.1600-0609.1977.tb02342.x.
Human lymphoid cells were examined by scanning electron microscopy (SEM) to see if a correlation existed between surface morphologic features and the presence of various surface markers and receptors. When viewed by SEM thymocytes appeared as smooth-surfaced cells with few surface microvilli; peripheral blood lymphocytes (PBL) on the other hand were moderately to densely villate with no entirely smooth-surfaced cells observed. Surface morphology within PBL samples was not uniform, due mainly to variations in the shape and number of microvilli. However, 2 distinctive types of surface morphology (termed Types 1 and 2) were discernable with a small number of cells displaying features of both groups (Type 3). The majority of E-rosette forming cells (T lymphocytes) displayed Type 1 and the majority of cells bearing demonstrable surface immunoglobulin (B lymphocytes) displayed Type 2 morphology. Exposure of PBL to anti-T cell specific ALG resulted in cytolysis of cells with Type 1 morphology while cells with Type 2 morphology appeared largely unaffected. PBL with Fc and C3 receptors displayed all 3 types of morphology. It is concluded that T and B lymphocytes do have subtle but nevertheless discernable differences in surface morphology and within these 2 groups, variations in surface morphology are probably associated with changes in the physiological status of the cell.
通过扫描电子显微镜（SEM）对人类淋巴细胞进行检查，以确定表面形态特征与各种表面标志物和受体的存在之间是否存在相关性。在扫描电子显微镜下观察时，胸腺细胞表现为表面光滑、几乎没有表面微绒毛的细胞；另一方面，外周血淋巴细胞（PBL）则有中度至密集的绒毛，未观察到完全表面光滑的细胞。PBL样本中的表面形态并不均匀，主要是由于微绒毛的形状和数量存在差异。然而，可以辨别出两种独特的表面形态类型（称为1型和2型），少数细胞同时显示出两组的特征（3型）。大多数E花环形成细胞（T淋巴细胞）表现为1型，大多数带有可检测表面免疫球蛋白的细胞（B淋巴细胞）表现为2型形态。将PBL暴露于抗T细胞特异性抗淋巴细胞球蛋白（ALG）会导致1型形态的细胞发生细胞溶解，而2型形态的细胞基本不受影响。具有Fc和C3受体的PBL表现出所有三种形态类型。结论是，T淋巴细胞和B淋巴细胞在表面形态上确实存在细微但可辨别的差异，并且在这两组细胞中，表面形态的变化可能与细胞生理状态的改变有关。
