Cuschieri A, Mughal S
J Anat. 1985 Jan;140 ( Pt 1)(Pt 1):93-104.
Human peripheral blood lymphocytes were cultured with phytohaemagglutinin or pokeweed mitogen for various intervals up to 17 days and studied by scanning electron microscopy. Activated lymphocytes in 3 day cultures were large irregular cells characterised mainly by an abundance of very fine microvilli, which were much thinner, shorter and more densely packed than the microvilli on uncultured lymphocytes. Cells intermediate in size and surface morphology between these and unstimulated lymphocytes were numerous in 1 day cultures. Some motile cells and large cells with finger-like or conical microvilli were also present. Cell counts showed that after 6 days in phytohaemagglutinin culture small villous cells resembling normal healthy lymphocytes were progressively more numerous, suggesting that most of the activated cells reverted to small lymphocytes. Very large cells were also present and displayed a heterogeneous surface morphology of villi, ridges, blebs and ruffles. A few of these cells had typical monocytoid features but others were predominantly villous. In pokeweed mitogen cultures there was extensive cellular degeneration affecting all cell types after 6 days. In unstimulated cultures the lymphocytes had fewer microvilli or were smooth-surfaced. There was extensive lymphocyte degeneration after 3 days and eventually typical monocytes were the predominant cells. Large, villous cells, which could be activated lymphocytes, were occasionally encountered in unstimulated cultures.
将人外周血淋巴细胞用植物血凝素或商陆有丝分裂原培养不同时间，最长培养17天，然后通过扫描电子显微镜进行研究。在3天培养物中的活化淋巴细胞是大的不规则细胞，其主要特征是有大量非常细的微绒毛，这些微绒毛比未培养淋巴细胞上的微绒毛更细、更短且更密集。在1天培养物中，大小和表面形态介于这些细胞与未刺激淋巴细胞之间的细胞很多。还存在一些活动细胞以及带有指状或锥形微绒毛的大细胞。细胞计数显示，在植物血凝素培养6天后，类似于正常健康淋巴细胞的小绒毛状细胞逐渐增多，这表明大多数活化细胞恢复为小淋巴细胞。也存在非常大的细胞，其表面形态为绒毛、嵴、泡和褶皱的异质性。这些细胞中有一些具有典型的单核细胞样特征，但其他细胞主要是绒毛状的。在商陆有丝分裂原培养物中，6天后所有细胞类型都出现广泛的细胞变性。在未刺激的培养物中，淋巴细胞的微绒毛较少或表面光滑。3天后出现广泛的淋巴细胞变性，最终典型的单核细胞成为主要细胞。在未刺激的培养物中偶尔会遇到大的、绒毛状细胞，它们可能是活化的淋巴细胞。
