Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, Taiwan.
Department of Agronomy, National Chung Hsing University, Taichung 40227, Taiwan; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40402, Taiwan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jan 1;1104:81-88. doi: 10.1016/j.jchromb.2018.11.006. Epub 2018 Nov 6.
Salvia miltiorrhiza has been widely used in Asia for medicinal purposes for >1000 years due to the high levels of bioactive constituents it contains. In this study, a simple and rapid ultrasound-assisted liquid extraction (5 min) was applied for the extraction of these bioactive constituents. The extracts were analyzed by using rapid ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with simultaneous positive and negative electrospray ionization in a single analytical run. Eight analytes were separated within 2.2 min during 6 min of run time with UHPLC-MS/MS operated in multiple reaction monitoring (MRM) mode. The concentration of salvianolic acids and tanshinones in the different organs of different varieties of Salvia miltiorrhiza ranged from 6.4 to 382.1 mg/g and 0.03 to 31.7 mg/g, respectively. To the best of our knowledge, this is the first report to characterize the tanshinone compounds found in the flower and stem/leaf of Salvia miltiorrhiza by UHPLC-MS/MS.
丹参在亚洲被广泛用于药用已有 1000 多年的历史，这是由于其含有高水平的生物活性成分。在这项研究中，应用了一种简单快速的超声辅助液-液萃取（5 分钟）来提取这些生物活性成分。采用快速超高效液相色谱-串联质谱（UHPLC-MS/MS），在单次分析运行中同时进行正、负离子电喷雾电离，对提取物进行了分析。在 6 分钟的运行时间内，8 种分析物在 2.2 分钟内得到分离，UHPLC-MS/MS 以多反应监测（MRM）模式运行。不同品种丹参不同器官中丹酚酸和丹参酮的浓度范围分别为 6.4 至 382.1 mg/g 和 0.03 至 31.7 mg/g。据我们所知，这是首次采用 UHPLC-MS/MS 对丹参花和茎/叶中发现的丹参酮化合物进行特征描述的报道。
