Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Phytochem Anal. 2011 May-Jun;22(3):247-57. doi: 10.1002/pca.1272. Epub 2010 Nov 2.
The increasing demands of roots and rhizomes of Salvia miltiorrhiza almost exhausted the wild Salvia sources in China. However, the content and composition of phenolic acids in the aerial parts of the plant and their potential to be used as a substitute has not been explored.
To evaluate the potential of the aerial parts of Salvia miltiorrhiza as new natural sources of phenolic acids.
HPLC coupled with diode array detection (DAD) and electrospray ionization multistage mass spectrometry (ESI/MS(n) ) has been used for qualitative and quantitative analysis of phenolic compounds.
A total of 38 phenolic compounds were identified or tentatively characterized. A quantitative HPLC-DAD method allowing the simultaneously quantification of six phenolic acids was optimized and validated for linearity, precision, accuracy, and limits of detection and quantification. Calibration curves showed good linear regression (r² > 0.9991) within test ranges; the recoveries ranged between 95.64 and 101.67% and the RSDs were less than 3.01%.
The developed methods have been proved to be effective for the identification and quantification of phenolic acids in S. miltiorrhiza. The results obtained suggest that the aerial parts of the plant could be used as an alternative source of sage phenolics.
丹参的根和根茎需求不断增加，几乎耗尽了中国的野生丹参资源。然而，该植物地上部分的酚酸含量和组成及其作为替代品的潜力尚未得到探索。
评估丹参地上部分作为新的天然酚酸资源的潜力。
采用高效液相色谱法（HPLC）与二极管阵列检测（DAD）和电喷雾电离多级质谱（ESI/MS(n)）联用对酚类化合物进行定性和定量分析。
共鉴定或初步表征了 38 种酚类化合物。建立了一种同时定量测定 6 种酚酸的 HPLC-DAD 方法，并对其进行了线性、精密度、准确度、检测限和定量限的验证。校准曲线在测试范围内具有良好的线性回归（r²>0.9991）；回收率在 95.64%至 101.67%之间，RSD 小于 3.01%。
所建立的方法已被证明可有效用于鉴定和定量分析丹参中的酚酸。研究结果表明，该植物地上部分可作为鼠尾草酚类物质的替代来源。
