CIC biomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, 20014 San Sebastián, Spain.
Ikerbasque, Basque Foundation for Science, Mª Díaz de Haro 3, 48013 Bilbao, Spain.
Biosensors (Basel). 2018 Nov 15;8(4):110. doi: 10.3390/bios8040110.
Among all new nanomaterials, metal nanoclusters (NCs) have attracted special attention due to their interesting optical properties, among others. Metal NCs have been recently studied and used as sensors for different analytes. However, there is a need to explore the potential of these new sensors in a systematic manner and to develop new systems to broaden the possibilities that sensing offers to the industry. In this work, we show the potential use of repeat protein scaffolds as versatile templates for the synthesis and stabilization of various metal NCs, specifically Au, Ag, and CuNCs. The resulting protein-metal NCs hybrids are evaluated as sensors for different stimuli such as temperature, ions, or reactive oxygen species (ROS). Among the three protein-metal NCs, all performed nicely as temperature sensors, AuNCs responded to metal ions, and AgNCs were able to detect ROS.
在所有新型纳米材料中，金属纳米团簇（NCs）因其有趣的光学性质等而受到特别关注。金属 NCs 最近已被研究并用作不同分析物的传感器。然而，需要以系统的方式探索这些新型传感器的潜力，并开发新系统以拓宽传感为工业带来的可能性。在这项工作中，我们展示了重复蛋白支架作为合成和稳定各种金属 NCs（特别是 Au、Ag 和 CuNCs）的通用模板的潜在用途。所得的蛋白-金属 NCs 杂化物被评估为对温度、离子或活性氧物种（ROS）等不同刺激的传感器。在这三种蛋白-金属 NCs 中，所有作为温度传感器都表现良好，AuNCs 对金属离子有响应，而 AgNCs 能够检测 ROS。
