Somta Prakit, Jomsangawong Achara, Yundaeng Chutintorn, Yuan Xingxing, Chen Jingbin, Tomooka Norihiko, Chen Xin
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Genes (Basel). 2018 Nov 15;9(11):555. doi: 10.3390/genes9110555.
The azuki bean weevil ( L.) is an insect pest responsible for serious postharvest seed loss in leguminous crops. In this study, we performed quantitative trait locus (QTL) mapping of seed resistance to in moth bean ( [Jaqc.] Maréchal). An F₂ population of 188 plants developed by crossing resistant accession 'TN67' (wild type from India; male parent) and susceptible accession 'IPCMO056' (cultivated type from India; female parent) was used for mapping. Seeds of the F₂ population from 2014 and F₃ populations from 2016 and 2017 were bioassayed with , and the percentage of damaged seeds and progress of infestation severity were measured. Segregation analysis suggested that resistance in TN176 is controlled by a single dominant gene, designated as . QTL analysis revealed one principal and one modifying QTL for the resistance, named and , respectively. was located on linkage group 2 between simple sequence repeat markers CEDG261 and DMB-SSR160 and accounted for 50.41% to 64.23% of resistance-related traits, depending on the trait and population. Comparative genomic analysis suggested that is the same as QTL conferring resistance in wild azuki bean ( Tateishi and Maxted). Markers CEDG261 and DMB-SSR160 should be useful for marker-assisted selection for resistance in moth bean.
赤小豆象（L.）是一种害虫，会导致豆科作物收获后种子严重损失。在本研究中，我们对蛾豆（[Jaqc.] Maréchal）种子对赤小豆象的抗性进行了数量性状基因座（QTL）定位。通过将抗性种质‘TN67’（来自印度的野生型；父本）和感病种质‘IPCMO056’（来自印度的栽培型；母本）杂交，构建了一个包含188株植株的F₂群体用于定位。对2014年F₂群体的种子以及2016年和2017年F₃群体的种子进行了赤小豆象生物测定，并测量了受损种子的百分比和侵染严重程度的进展。分离分析表明，TN176中的赤小豆象抗性由一个单显性基因控制，命名为 。QTL分析揭示了一个主要抗性QTL和一个修饰QTL，分别命名为 和 。 位于连锁群2上，在简单序列重复标记CEDG261和DMB - SSR160之间，根据性状和群体不同，它解释了与抗性相关性状的50.41%至64.23%。比较基因组分析表明， 与野生赤小豆（Tateishi和Maxted）中赋予赤小豆象抗性的QTL 相同。标记CEDG261和DMB - SSR160应有助于蛾豆赤小豆象抗性的标记辅助选择。
