Genetic Dissection of Azuki Bean Weevil (Callosobruchus chinensis L.) Resistance in Moth Bean (Vigna aconitifolia [Jaqc.] Maréchal).

The azuki bean weevil ( L.) is an insect pest responsible for serious postharvest seed loss in leguminous crops. In this study, we performed quantitative trait locus (QTL) mapping of seed resistance to in moth bean ( [Jaqc.] Maréchal). An F₂ population of 188 plants developed by crossing resistant accession 'TN67' (wild type from India; male parent) and susceptible accession 'IPCMO056' (cultivated type from India; female parent) was used for mapping. Seeds of the F₂ population from 2014 and F₃ populations from 2016 and 2017 were bioassayed with , and the percentage of damaged seeds and progress of infestation severity were measured. Segregation analysis suggested that resistance in TN176 is controlled by a single dominant gene, designated as . QTL analysis revealed one principal and one modifying QTL for the resistance, named and , respectively. was located on linkage group 2 between simple sequence repeat markers CEDG261 and DMB-SSR160 and accounted for 50.41% to 64.23% of resistance-related traits, depending on the trait and population. Comparative genomic analysis suggested that is the same as QTL conferring resistance in wild azuki bean ( Tateishi and Maxted). Markers CEDG261 and DMB-SSR160 should be useful for marker-assisted selection for resistance in moth bean.