酵母激活剂的作用机制：GAL4衍生物对哺乳动物TFIID-启动子相互作用的直接影响

Mechanism of action of a yeast activator: direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions.

作者信息

Horikoshi M, Carey M F, Kakidani H, Roeder R G

机构信息

Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.

出版信息

Cell. 1988 Aug 26;54(5):665-9. doi: 10.1016/s0092-8674(88)80011-4.

DOI:10.1016/s0092-8674(88)80011-4

PMID:3044608

Abstract

We have analyzed interactions between the mammalian TATA factor (TFIID) and derivatives of the yeast activator GAL4. The interaction of the TATA factor on the adenovirus E4 promoter with GAL4 binding sites adjacent to the TATA site was qualitatively altered in response to GAL4 binding. Alterations in the TFIID interactions were observed with two GAL4 derivatives that stimulated hybrid E4 promoter activity in vitro but not with a third derivative that bound to DNA but showed no activation. These results indicate that TFIID is a direct target for a GAL4 activation domain and suggest a simple general model for the activation mechanism.

摘要

我们分析了哺乳动物TATA因子（TFIID）与酵母激活因子GAL4衍生物之间的相互作用。TATA因子在腺病毒E4启动子上与TATA位点相邻的GAL4结合位点的相互作用，会因GAL4的结合而发生定性改变。在两种GAL4衍生物中观察到TFIID相互作用的改变，这两种衍生物在体外刺激了杂交E4启动子活性，但在第三种与DNA结合但无激活作用的衍生物中未观察到这种改变。这些结果表明TFIID是GAL4激活域的直接靶点，并提出了一个简单的激活机制通用模型。