羟磷灰石-细菌纳米纤维素支架的制备及其纤维素纳米晶的辅助作用。
Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals.
机构信息
Biotechnology of Biopolymers and Bioactive Compounds Special Research Unit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biotechnology of Biopolymers and Bioactive Compounds Special Research Unit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand; Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
出版信息
Carbohydr Polym. 2019 Feb 1;205:159-166. doi: 10.1016/j.carbpol.2018.10.034. Epub 2018 Oct 12.
Bacterial nanocellulose/hydroxyapatite/cellulose nanocrystal (BHC) composites were synthesized via in-situ synthesis using cellulose nanocrystals (CNCs) to improve colloidal stability and the dispersion of hydroxyapatite (HA) during the bacterial nanocellulose (BNC) cultivation period. Transmission electron microscopy images and energy dispersive spectroscopy (EDS) results confirmed the dispersion of HA on the CNC particles with a Ca/P ratio of 1.66 corresponding to that of the stoichiometric HA. The SEM images and EDS results showed that the integration of the HA and BNC network without CNC assistance (BHA (0.25 and 0.5 wt.%) composites) was less than that for BHC at both concentrations. Fourier-transform infrared analysis, XRD and thermal degradation revealed the effect of HA on the BHC composites with a decreased CrI% and improved thermal property. Cytotoxicity proved the potential for using BHC composites for bone tissue engineering scaffold with cell viability up to 83.4 ± 3.6% compared to the negative control (99.2 ± 0.08%).
细菌纳米纤维素/羟基磷灰石/纤维素纳米晶体（BHC）复合材料是通过原位合成制备的，使用纤维素纳米晶体（CNCs）提高了胶体稳定性和羟基磷灰石（HA）在细菌纳米纤维素（BNC）培养期间的分散性。透射电子显微镜图像和能谱（EDS）结果证实了 HA 在 CNC 颗粒上的分散，其 Ca/P 比为 1.66，对应于化学计量的 HA。SEM 图像和 EDS 结果表明，在没有 CNC 辅助的情况下（BHA（0.25 和 0.5wt.%）复合材料），HA 与 BNC 网络的集成度低于两种浓度下的 BHC。傅里叶变换红外分析、XRD 和热降解表明，HA 对 BHC 复合材料的影响导致 CrI%降低，热性能得到改善。细胞毒性证明了 BHC 复合材料用于骨组织工程支架的潜力，细胞活力高达 83.4±3.6%，与阴性对照（99.2±0.08%）相比。
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