Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Wuhan HVSEN Biotechnology Co., Ltd., Zhangbai Road, Dongxihu District, Wuhan, 430042, PR China.
Carbohydr Polym. 2019 Feb 1;205:540-549. doi: 10.1016/j.carbpol.2018.10.057. Epub 2018 Oct 25.
Dendritic cells (DCs) are professional antigen-presenting cells (APC) that play a central role in the initiation and regulation of immune responses. We have previously demonstrated that Lycium barbarum polysaccharides liposomes (LBPL) as immune adjuvant elicits strong antigen-specific Th1 immune responses. The purpose of this study was to investigate underlying mechanism of liposomes promoting effect of Lycium barbarum polysaccharides (LBP) on activating DCs. LBP were loaded with high entrapment efficiency (86%) into liposomes using reverse phase evaporation. LBPL activation of phenotypic and functional maturation of DCs was explored through mechanistic studies of the TLR4-MyD88-NF-κB signaling pathway and amount of proinflammatory cytokines released. We found that LBPL indeed activated immature DCs and induced DCs maturation characterized by up-regulation of co-stimulatory molecules (MHCII, CD80, CD86), production of cytokines (IL-12p40, TNF-α), and enhancement of antigen uptake. Additionally, we demonstrated that liposomes could promote LBP up-regulation of TLR4, MyD88, TRAF6, NF-κB gene and protein expression.
树突状细胞 (DCs) 是专业的抗原提呈细胞 (APC)，在免疫反应的启动和调节中发挥核心作用。我们之前的研究表明，枸杞多糖脂质体 (LBPL) 作为免疫佐剂可引发强烈的抗原特异性 Th1 免疫反应。本研究旨在探讨脂质体促进枸杞多糖 (LBP) 激活 DCs 的潜在机制。采用反相蒸发法将高包封率 (86%) 的 LBP 载入脂质体。通过 TLR4-MyD88-NF-κB 信号通路和促炎细胞因子释放量的研究，探讨 LBPL 对 DCs 表型和功能成熟的激活作用。结果发现，LBPL 确实能激活未成熟的 DCs，并诱导 DCs 成熟，其特征是共刺激分子 (MHCII、CD80、CD86) 的上调、细胞因子 (IL-12p40、TNF-α) 的产生以及抗原摄取能力的增强。此外，我们还证明了脂质体可以促进 LBP 上调 TLR4、MyD88、TRAF6 和 NF-κB 基因和蛋白的表达。
