School of Medical and Health Sciences, Edith Cowan University, Joondalup, 6027, Australia; School of Population and Global Health, University of Western Australia, Nedlands, 6009, Australia.
Department of Immunology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, 02-096, Poland.
Immunobiology. 2019 Jan;224(1):110-115. doi: 10.1016/j.imbio.2018.10.002. Epub 2018 Oct 19.
Increased body fat may be associated with an increased risk of developing an underlying pro-inflammatory state, thus leading to greater risk of developing certain chronic conditions. Immunoglobulin G has the ability to exert both anti- and pro-inflammatory effects, and the N-glycosylation of the fragment crystallisable portion is involved in mediating this process. Body mass index, a rudimentary yet gold standard indication for body fat, has been shown to be associated with agalactosylated immunoglobulin G N-glycans.
We aimed to determine the association between increased body fat and the immunoglobulin G glycosylation features, comparing body mass index to other measures of body fat distribution.
We investigated a sample of 637 community-based 45-69 year olds, with mixed phenotypes, residing in Busselton, Western Australia. Body mass index and the waist-to-hip and waist-to-height ratios were calculated using anthropometry, while dual-energy x-ray absorptiometry was performed to gain an accurate measure of total and area specific body fat. Serum immunoglobulin GN-glycans were analysed by ultra-performance liquid chromatography.
Twenty-two N-glycan peaks were found to be associated with at least one of the fat measures. While the previous association of body mass index to agalactosylated immunoglobulin G was replicated, measures of central adiposity explained the most variation in the immunoglobulin G glycome.
Central adiposity is associated with an increased pro-inflammatory fraction of immunoglobulin G, suggesting that the android/gynoid ratio or waist-to-height ratio instead be considered when controlling for adiposity in immunoglobulin G glycome biomarker studies.
体脂肪增加可能与潜在的促炎状态风险增加有关，从而导致某些慢性疾病的风险增加。免疫球蛋白 G 具有抗炎和促炎作用，其片段结晶区的 N-糖基化参与了这一过程。体重指数是体脂肪的基本但标准的指标，已被证明与半乳糖化免疫球蛋白 G N-糖链有关。
我们旨在确定体脂肪增加与免疫球蛋白 G 糖基化特征之间的关联，并比较体重指数与其他体脂肪分布指标。
我们调查了 637 名居住在西澳大利亚州巴斯尔顿的 45-69 岁的社区混合表型人群。体重指数和腰臀比和腰高比是通过人体测量法计算的，而双能 X 射线吸收法则用于获得总脂肪和区域特定脂肪的准确测量值。血清免疫球蛋白 G N-糖链通过超高效液相色谱法进行分析。
发现 22 个 N-糖链峰与至少一种脂肪指标有关。虽然体重指数与半乳糖化免疫球蛋白 G 的先前关联得到了复制，但中心性肥胖的指标解释了免疫球蛋白 G 糖组中最多的变化。
中心性肥胖与免疫球蛋白 G 的促炎部分增加有关，这表明在控制免疫球蛋白 G 糖组生物标志物研究中的肥胖时，应考虑安卓/臀围比或腰高比。
