Pflüger E, Lang E, Benke R, Heckl-Ostreicher B, Altevogt P, Schirrmacher V
Institut für Immunologie und Genetik, Deutsches Krebsforschungszentrum, Heidelberg, F.R. Germany.
Clin Exp Metastasis. 1988 Nov-Dec;6(6):485-99. doi: 10.1007/BF01784379.
Tumor cell variants which grow adherent to a plastic surface could be isolated in a reproducible way from the high metastatic tumor cell line ESb which grows in a suspension culture. This occurred when starting selection from the uncloned parental line as well as from a freshly derived non-adhesive subclone. The variants showed changes in their karyotype. These were quantitative (tetraploidization) and qualitative (single chromosome aberrations involving the chromosomes 12 and 17 and a marker MX-7). Phenotypic cell surface changes were documented in vitro by immunofluorescence using a monoclonal antibody (mAb 12-15) directed against a distinct plasma membrane glycoprotein of 60-69kD (gp 60-69). The expression of gp 60-69 increased with time of selection for adherence to plastic surface. The adherent cells showed in all cases a greatly reduced overall malignancy as seen by a prolonged survival time of respective tumor bearing animals compared with the suspension growing parental cells.
能够附着于塑料表面生长的肿瘤细胞变体，可以以可重复的方式从悬浮培养生长的高转移性肿瘤细胞系ESb中分离出来。当从未克隆的亲代细胞系以及新衍生的非黏附性亚克隆开始筛选时，就会出现这种情况。这些变体的核型发生了变化。这些变化包括数量上的（四倍体化）和质量上的（涉及12号和17号染色体以及一个标记MX-7的单条染色体畸变）。使用针对一种60 - 69kD独特质膜糖蛋白（gp 60 - 69）的单克隆抗体（mAb 12 - 15），通过免疫荧光在体外记录了表型细胞表面变化。gp 60 - 69的表达随着选择附着于塑料表面的时间而增加。与悬浮生长的亲代细胞相比，通过相应荷瘤动物延长的存活时间可以看出，在所有情况下，贴壁细胞的总体恶性程度都大大降低。
