Pot-Deprun J, Poupon M F, Sweeney F L, Chouroulinkov I
J Natl Cancer Inst. 1983 Dec;71(6):1241-5.
Karyotype patterns, growth, metastasis, and immunogenicity were compared in a rhabdomyosarcoma induced by a single injection of metallic nickel with those in subsequent tumor-cloned cell lines. The primary tumor was induced in a male WAG rat by im injection of 20 mg nickel powder. The parental cell line (9-4/0) and 8 cell subpopulations (J 9-4) isolated from a primary tumor by cloning on agarose were examined. The tumor cell dose inducing tumors in 50% of the animals after sc injection (TD50) and the in vitro growth characteristics showed a marked heterogeneity between parental and cloned tumor cell lines. In vitro doubling times and saturation densities were also heterogeneous without showing a discernible relationship with TD50. Chromosome patterns of the cell lines exhibited very similar modal numbers, whereas chromosome numbers were somewhat different; neither of these exhibited any correlation with tumorigenicity. Parental cell line 9-4/0 expressed a significant degree of immunogenicity, but it did not protect against pulmonary metastasis in immunized rats. Among 6 clones studied, only clone J 9-4/2 appeared to be immunogenic and reduced metastatic spread. The relevance of the comparison between the different characteristics is discussed.
将单次注射金属镍诱导的横纹肌肉瘤的核型模式、生长、转移和免疫原性与后续肿瘤克隆细胞系进行了比较。通过皮下注射20mg镍粉在雄性WAG大鼠中诱导原发性肿瘤。检查了通过在琼脂糖上克隆从原发性肿瘤中分离出的亲本细胞系（9-4/0）和8个细胞亚群（J 9-4）。皮下注射后在50%的动物中诱导肿瘤的肿瘤细胞剂量（TD50）和体外生长特性显示亲本和克隆肿瘤细胞系之间存在明显的异质性。体外倍增时间和饱和密度也存在异质性，与TD50没有明显关系。细胞系的染色体模式显示出非常相似的众数，而染色体数量有所不同；这些均与致瘤性没有任何相关性。亲本细胞系9-4/0表现出显著程度的免疫原性，但它不能保护免疫大鼠免受肺转移。在所研究
的6个克隆中，只有克隆J 9-4/2似乎具有免疫原性并减少了转移扩散。讨论了不同特征之间比较的相关性。
