Akdeniz University, Medical School, Department of Biophysics, Antalya, Turkey.
Akdeniz University, Medical School, Department of Biophysics, Antalya, Turkey.
Food Chem Toxicol. 2019 Jan;123:453-458. doi: 10.1016/j.fct.2018.11.021. Epub 2018 Nov 15.
Sulfite is a commonly used preservative in food products, alcoholic beverages and pharmaceutical products. We investigated the effect of sulfite, on locomotor activity as well as the relationship of these effects with oxidant and antioxidant capacities, cPLA2 enzyme activity. Thirty male Wistar albino rats were randomly divided into two groups as control(C) and sulfite(S). Animals in the S group were given freshly prepared sulfite for 35 days via gastric gavage (100 mg/kg/day) while the C group received equal volumes of distilled water via gavage for the same period. Open-field tests were performed to all groups and animals were sacrificed. Total antioxidant capacity(TAC), TBARS levels, cPLA2 activity as well as amount of caspase-3 positive cells were analyzed on the hippocampi. In the open field test, distance and velocity values of the S group increased with respect to controls. TBARS and cPLA2 activity were also increased in the S group, while levels of TAC decreased compared to controls. Immunohistochemical analysis showed that sulfite ingestion caused an increase in the amount of hippocampal caspase-3 positive cells. In conclusion, sulfite seemed to increase locomotor activity. cPLA2 might play a role in ingested sulfite-induced oxidative stress and apoptotic cell death in the hippocampus.
亚硫酸盐是食品、酒精饮料和药物产品中常用的防腐剂。我们研究了亚硫酸盐对运动活动的影响，以及这些影响与氧化剂和抗氧化剂能力、cPLA2 酶活性的关系。30 只雄性 Wistar 白化大鼠随机分为两组：对照组（C）和亚硫酸盐组（S）。S 组动物通过胃灌胃（100mg/kg/天）给予新鲜制备的亚硫酸盐 35 天，而 C 组在相同时间内通过灌胃给予等量的蒸馏水。对所有组进行了旷场试验，然后处死动物。在海马体上分析总抗氧化能力（TAC）、TBARS 水平、cPLA2 活性以及 caspase-3 阳性细胞的数量。在旷场试验中，S 组的距离和速度值相对于对照组增加。S 组的 TBARS 和 cPLA2 活性也增加，而 TAC 水平与对照组相比降低。免疫组织化学分析表明，亚硫酸盐摄入导致海马 caspase-3 阳性细胞数量增加。总之，亚硫酸盐似乎增加了运动活动。cPLA2 可能在海马体中摄入的亚硫酸盐引起的氧化应激和细胞凋亡中起作用。
