Shao Heng, Gan Quan, Chen Zhuangfei, Zhu Shasha, Zhu Yanqing
Department of Geriatrics, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.
The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.
Evid Based Complement Alternat Med. 2022 May 29;2022:7761852. doi: 10.1155/2022/7761852. eCollection 2022.
Currently, there are many effective pharmacological treatments for generalized anxiety disorder (GAD), formulated herbal granule is also an alternative way. Our research intends to construct a pharmacological network on genetic targets and pathways between Jiu Wei Zhen Xin Formula (JWZXF) and GAD. Through the TCMSP database, we collected the active ingredients of JWZXF and potential targets of the active ingredients. The GAD-related proteins collected from GeneCards database and DisGeNET database were combined. Component-target protein networks were constructed and visualized using Cytoscape 3.8.2 software to comprehensively clarify the relationships between ingredients, components, and targets. The intersection targets were imported into the STRING database, and the protein-protein interaction (PPI) network was constructed. We constructed and analyzed the visualized "drug-target-disease" network. Gene Ontology (GO) enrichment together with Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted on the common target through R language. Forty-one effective components and 106 potential targets of JWZXF were found. There were top ten hub genes and multiple important signaling pathways involved in the treatment of GAD with the JWZXF. This study expounded the pharmacological actions and molecular mechanisms of the JWZXF in treating GAD from a holistic perspective. The potential pharmacological effects of the JWZXF are closely related to regulation because not only does it comprehensively analyze the possible mechanism of JWZXF treatment of GAD but it can also facilitate further in-depth research and provide a theoretical basis for the clinical expansion of its application.
目前，对于广泛性焦虑症（GAD）有许多有效的药物治疗方法，中药配方颗粒也是一种替代方法。我们的研究旨在构建一个关于九味镇心颗粒（JWZXF）与GAD之间遗传靶点和通路的药理网络。通过中药系统药理学数据库与分析平台（TCMSP）数据库，我们收集了JWZXF的活性成分和活性成分的潜在靶点。将从基因卡片（GeneCards）数据库和疾病基因数据库（DisGeNET）收集的GAD相关蛋白进行整合。使用Cytoscape 3.8.2软件构建并可视化成分-靶点蛋白网络，以全面阐明成分、组分和靶点之间的关系。将交集靶点导入STRING数据库，构建蛋白质-蛋白质相互作用（PPI）网络。我们构建并分析了可视化的“药物-靶点-疾病”网络。通过R语言对共同靶点进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）富集分析。发现JWZXF有41种有效成分和106个潜在靶点。有十大核心基因和多个重要信号通路参与JWZXF治疗GAD。本研究从整体角度阐述了JWZXF治疗GAD的药理作用和分子机制。JWZXF的潜在药理作用与调控密切相关，因为它不仅全面分析了JWZXF治疗GAD的可能机制，还能促进进一步深入研究，并为其临床应用拓展提供理论依据。
