Department of Biophysics, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Department of Biophysics, Faculty of Medicine, Hitit University, Çorum, Turkey.
Neurochem Res. 2022 Nov;47(11):3331-3343. doi: 10.1007/s11064-022-03684-z. Epub 2022 Jul 27.
In this study, the effects of different doses of sulfite on learning, memory, and long term potentiation as well as the relationship of these effects with acetylcholine pathways, Arc and synapsin 1 levels were investigated. Sixty male Wistar albino rats were randomly divided into three groups as control, S100, and S260. Sodiummetabisulfite (S100;100 mg/kg/day, S260;260 mg/kg/day) was given by oral administration. Behavioral changes were evaluated. After long term potentiation recordings from the perforant pathway-dentate gyrus synapses, animals were sacrificed. Acetylcholinesterase activity, choline acetyltransferase activity, acetylcholine level as well as Arc and Synapsin 1 expressions were analyzed on the hippocampi. The total distance and average velocity values in the open field and Morris water maze tests increased in the sulfite groups, while the discrimination index in the novel object recognition test decreased compared to controls. Acetylcholine levels and choline acetyltransferase activity were also increased in the sulfite groups, while acetylcholinesterase activity was decreased compared to controls. Sulfite intake attenuated long term potentiation in the hippocampus. It has been observed that the excitatory postsynaptic potential slope and population spike amplitude of the field potentials obtained in sulfite groups decreased. This impairment was accompanied by a decrease in Arc and synapsin 1 expressions. In conclusion, it has been shown that sulfite intake in adults impairs learning and memory, possibly mediated by the cholinergic pathway. It is considered that the decrement in Arc and synapsin expressions may play a role in the mechanism underlying the impairment in long term potentiation caused by toxicity.
在这项研究中，研究了不同剂量的亚硫酸盐对学习、记忆和长时程增强的影响，以及这些影响与乙酰胆碱途径、Arc 和突触素 1 水平的关系。将 60 只雄性 Wistar 白化大鼠随机分为三组：对照组、S100 组和 S260 组。通过口服给予偏亚硫酸钠（S100：100mg/kg/天，S260：260mg/kg/天）。评估行为变化。从穿通通路-齿状回突触记录长时程增强后，处死动物。分析海马中的乙酰胆碱酯酶活性、胆碱乙酰转移酶活性、乙酰胆碱水平以及 Arc 和突触素 1 的表达。与对照组相比，在开放场和 Morris 水迷宫测试中，亚硫酸盐组的总距离和平均速度值增加，而新物体识别测试中的辨别指数降低。亚硫酸盐组的乙酰胆碱水平和胆碱乙酰转移酶活性也增加，而乙酰胆碱酯酶活性降低。亚硫酸盐摄入可减弱海马中的长时程增强。观察到在亚硫酸盐组中获得的场电位的兴奋性突触后电位斜率和群体锋电位幅度降低。这种损伤伴随着 Arc 和突触素 1 表达的减少。总之，研究表明，成年人摄入亚硫酸盐会损害学习和记忆，这可能是通过胆碱能途径介导的。认为 Arc 和突触素表达的减少可能在毒性引起的长时程增强损伤的机制中起作用。
