IGEPP, INRA, AGROCAMPUS OUEST, Université Rennes, Domaine de la Motte, Le Rheu F-35653, France.
IGEPP, INRA, AGROCAMPUS OUEST, Université Rennes, Domaine de la Motte, Le Rheu F-35653, France.
Genomics. 2019 Dec;111(6):1629-1640. doi: 10.1016/j.ygeno.2018.11.013. Epub 2018 Nov 15.
Plasmodiophora brassicae is an obligate biotrophic pathogenic protist responsible for clubroot, a root gall disease of Brassicaceae species. In addition to the reference genome of the P. brassicae European e3 isolate and the draft genomes of Canadian or Chinese isolates, we present the genome of eH, a second European isolate. Refinement of the annotation of the eH genome led to the identification of the mitochondrial genome sequence, which was found to be bigger than that of Spongospora subterranea, another plant parasitic Plasmodiophorid phylogenetically related to P. brassicae. New pathways were also predicted, such as those for the synthesis of spermidine, a polyamine up-regulated in clubbed regions of roots. A P. brassicae pathway genome database was created to facilitate the functional study of metabolic pathways in transcriptomics approaches. These available tools can help in our understanding of the regulation of P. brassicae metabolism during infection and in response to diverse constraints.
根肿菌是一种专性生物营养型的致病原生动物，可引起芸薹属植物的根肿病。除了 P. brassicae 欧洲 e3 分离株的参考基因组和加拿大或中国分离株的草图基因组外，我们还展示了 eH 第二个欧洲分离株的基因组。对 eH 基因组注释的细化导致了线粒体基因组序列的鉴定，发现其比另一种与 P. brassicae 亲缘关系密切的植物寄生性根肿菌 Spongospora subterranea 的线粒体基因组更大。还预测了新的途径，例如 spermidine 的合成途径，多胺在根的肿大区域中上调。创建了 P. brassicae 途径基因组数据库，以促进在转录组学方法中对代谢途径的功能研究。这些可用的工具可以帮助我们理解 P. brassicae 在感染过程中以及响应各种限制时的新陈代谢的调节。
