Faculty of Biology, Technische Universität Dresden, 01062 Dresden, Germany.
Int J Mol Sci. 2022 Jun 4;23(11):6293. doi: 10.3390/ijms23116293.
Clubroot is one of the most economically significant diseases worldwide. As a result, many investigations focus on both curing the disease and in-depth molecular studies. Although the first transcriptome dataset for the clubroot disease describing the clubroot disease was published in 2006, many different pathogen-host plant combinations have only recently been investigated and published. Articles presenting -omics data and the clubroot pathogen as well as different host plants were analyzed to summarize the findings in the richness of these datasets. Although genome data for the protist have only recently become available, many effector candidates have been identified, but their functional characterization is incomplete. A better understanding of the life cycle is clearly required to comprehend its function. While only a few proteome studies and metabolome analyses were performed, the majority of studies used microarrays and RNAseq approaches to study transcriptomes. Metabolites, comprising chemical groups like hormones were generally studied in a more targeted manner. Furthermore, functional approaches based on such datasets have been carried out employing mutants, transgenic lines, or ecotypes/cultivars of either or other economically important host plants of the family. This has led to new discoveries of potential genes involved in disease development or in (partial) resistance or tolerance to . The overall contribution of individual experimental setups to a larger picture will be discussed in this review.
根肿病是全球最重要的经济作物病害之一。因此，许多研究都致力于治疗该疾病和深入的分子研究。尽管 2006 年发表了第一个描述根肿病的转录组数据集，但最近才对许多不同的病原体-宿主植物组合进行了研究和发表。本文分析了描述组学数据和根肿病病原体以及不同宿主植物的文章，以总结这些数据集的丰富性。尽管最近才获得了该原生动物的基因组数据，但已经鉴定出许多效应子候选物，但它们的功能特征尚不完全清楚。显然，需要更好地了解其生命周期，以理解其功能。虽然仅进行了少数蛋白质组研究和代谢组分析，但大多数研究都使用微阵列和 RNAseq 方法来研究转录组。包含激素等化学基团的代谢物通常以更具针对性的方式进行研究。此外，还基于此类数据集进行了功能研究，使用了突变体、转基因系或 或其他经济上重要的 科宿主植物的生态型/品种。这导致了对参与疾病发展或（部分）抗性或耐受性的潜在基因的新发现。本文将讨论各个实验设置对整体的贡献。
