Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthansgatan 3-5, FI-20500 Åbo, Finland.
Centre for Functional Materials, Laboratory of Cell Biology, Åbo Akademi University, Artillerigatan 6, Åbo FI-20520, Finland.
Colloids Surf B Biointerfaces. 2019 Feb 1;174:136-144. doi: 10.1016/j.colsurfb.2018.10.064. Epub 2018 Oct 29.
In this study hierarchically-structured latex polymer coatings and self-supporting films were characterised and their suitability for cell growth studies was tested with Human Dermal Fibroblasts (HDF). Latex can be coated or printed on rigid or flexible substrates thus enabling high-throughput fabrication. Here, coverslip glass substrates were coated with blends of two different aqueous latex dispersions: hydrophobic polystyrene (PS) and hydrophilic carboxylated acrylonitrile butadiene styrene (ABS). The nanostructured morphology and topography of the latex films was controlled by varying the mixing ratio of the components in the latex blend. Thin latex-coatings retain high transparency on glass allowing optical and high resolution imaging of cell growth and morphology. Compared to coverslip glass surfaces and commercial well-plates HDF cell growth was enhanced up to 150-250 % on latex surfaces with specific nanostructure. Growth rates were correlated with selected roughness parameters such as effective surface area (S), RMS-roughness (S) and correlation length (S). High-resolution confocal microscopy clearly indicated less actin stress-fibre development in cells on the latex surface compared to coverslip glass. The results show that surface nanotopography can, by itself, passively modulate HDF cell proliferation and cytoskeletal architecture.
在这项研究中，我们对分层乳胶聚合物涂层和自支撑膜进行了表征，并使用人真皮成纤维细胞（HDF）测试了它们在细胞生长研究中的适用性。乳胶可以涂覆或印刷在刚性或柔性基底上，从而实现高通量制造。在这里，盖玻片玻璃基底涂覆有两种不同的水性乳胶分散体的混合物：疏水性聚苯乙烯（PS）和亲水性羧基丙烯腈丁二烯苯乙烯（ABS）。乳胶膜的纳米结构形态和形貌通过改变乳胶混合物中各成分的混合比来控制。薄的乳胶涂层在玻璃上保持高透明度，允许对细胞生长和形态进行光学和高分辨率成像。与盖玻片玻璃表面和商用微孔板相比，在具有特定纳米结构的乳胶表面上，HDF 细胞的生长可提高 150-250%。生长速率与选定的粗糙度参数相关，例如有效表面积（S）、均方根粗糙度（S）和相关长度（S）。高分辨率共聚焦显微镜清楚地表明，与盖玻片玻璃相比，乳胶表面上的细胞中肌动蛋白应力纤维的发育较少。结果表明，表面纳米形貌本身可以被动调节 HDF 细胞的增殖和细胞骨架结构。
