Laboratory of Natural Materials Technology, Åbo Akademi University, 20540 Turku, Finland.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, Guangdong, People's Republic of China.
Biomacromolecules. 2020 Apr 13;21(4):1560-1567. doi: 10.1021/acs.biomac.0c00107. Epub 2020 Mar 16.
Biodegradable and renewable materials, such as cellulose nanomaterials, have been studied as a replacement material for traditional plastics in the biomedical field. Furthermore, in chronic wound care, modern wound dressings, hydrogels, and active synthetic extracellular matrices promoting tissue regeneration are developed to guide cell growth and differentiation. Cells are guided not only by chemical cues but also through their interaction with the surrounding substrate and its physicochemical properties. Hence, the current work investigated plant-based cellulose nanomaterials and their surface characteristic effects on human dermal fibroblast (HDF) behavior. Four thin cellulose nanomaterial-based coatings produced from microfibrillar cellulose (MFC), cellulose nanocrystals (CNC), and two TEMPO-oxidized cellulose nanofibers (CNF) with different total surface charge were characterized, and HDF viability and adhesion were evaluated. The highest viability and most stable adhesion were on the anionic CNF coating with a surface charge of 1.14 mmol/g. On MFC and CNC coated surfaces, HDFs sedimented but were unable to anchor to the substrate, leading to low viability.
可生物降解和可再生材料，如纤维素纳米材料，已被研究作为传统塑料在生物医学领域的替代材料。此外，在慢性伤口护理中，现代伤口敷料、水凝胶和促进组织再生的活性合成细胞外基质被开发出来，以指导细胞的生长和分化。细胞不仅受到化学信号的引导，还受到与周围基质及其物理化学性质相互作用的引导。因此，目前的工作研究了基于植物的纤维素纳米材料及其表面特性对人皮肤成纤维细胞（HDF）行为的影响。四种基于微原纤维纤维素（MFC）、纤维素纳米晶体（CNC）和两种具有不同总表面电荷的 TEMPO 氧化纤维素纳米纤维（CNF）的薄纤维素纳米材料涂层被进行了表征，并评估了 HDF 的活力和黏附性。带 1.14mmol/g 表面电荷的阴离子 CNF 涂层具有最高的活力和最稳定的黏附性。在 MFC 和 CNC 涂层表面，HDF 沉淀但无法附着到基质上，导致活力较低。
