Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
Virology. 2019 Jan 2;526:222-230. doi: 10.1016/j.virol.2018.10.025. Epub 2018 Nov 14.
A product translated from the 1b gene of raspberry bushy dwarf virus (RBDV) was specifically detected in RBDV-infected Nicotiana benthamiana plants by immunoblot analysis. To analyze the effects of the 1b gene on virus infection in host plants, an RBDV deletion mutant virus (RB∆1bstop), which is unable to express the 1b gene, was constructed and inoculated to N. benthamiana plants. The results showed that accumulation of the virus genomic (g) RNAs 1 and 2 decreased in inoculated leaves, and that systemic virus spread was delayed compared with wild-type RBDV. In contrast, accumulation of the viral gRNAs 1 and 2 was elevated in RB∆1bstop-infected leaf tissues during ectopic expression of the 1b gene. Furthermore, we found that the 1b has weak RNA silencing suppressor activity.
通过免疫印迹分析，在感染了李痘病毒（RBDV）的菸草植株中特异性检测到李痘病毒 1b 基因的翻译产物。为了分析 1b 基因对宿主植物中病毒感染的影响，构建了无法表达 1b 基因的 RBDV 缺失突变病毒（RB∆1bstop），并将其接种到菸草植株上。结果表明，与野生型 RBDV 相比，接种叶片中病毒基因组（g）RNAs1 和 2 的积累减少，系统病毒传播延迟。相比之下，在异位表达 1b 基因时，RB∆1bstop 感染的叶片组织中病毒 gRNAs1 和 2 的积累增加。此外，我们发现 1b 具有较弱的 RNA 沉默抑制活性。
