从哈萨克斯坦红树莓中分离的树莓丛枝矮化病毒的遗传特征。

Genetic Characterization of Raspberry Bushy Dwarf Virus Isolated from Red Raspberry in Kazakhstan.

机构信息

Laboratory of Molecular Biology, Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan.

Department of Molecular Biology and Genetics, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.

出版信息

Viruses. 2023 Apr 16;15(4):975. doi: 10.3390/v15040975.

DOI:10.3390/v15040975

PMID:37112955

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10143182/

Abstract

Raspberry bushy dwarf virus (RBDV) is an economically significant pathogen of raspberry and grapevine, and it has also been found in cherry. Most of the currently available RBDV sequences are from European raspberry isolates. This study aimed to sequence genomic RNA2 of both cultivated and wild raspberry in Kazakhstan and compare them to investigate their genetic diversity and phylogenetic relationships, as well as to predict their protein structure. Phylogenetic and population diversity analyses were performed on all available RBDV RNA2, MP and CP sequences. Nine of the isolates investigated in this study formed a new, well-supported clade, while the wild isolates clustered with the European isolates. Predicted protein structure analysis revealed two regions that differed between α- and β-structures among the isolates. For the first time, the genetic composition of Kazakhstani raspberry viruses has been characterized.

摘要

树莓丛枝病毒（RBDV）是 raspberry 和葡萄的重要经济病原，在樱桃中也有发现。目前大多数可用的 RBDV 序列来自欧洲 raspberry 分离株。本研究旨在对哈萨克斯坦栽培和野生 raspberry 的基因组 RNA2 进行测序，并对其进行比较，以调查其遗传多样性和系统发育关系，并预测其蛋白结构。对所有可用的 RBDV RNA2、MP 和 CP 序列进行了系统发育和种群多样性分析。本研究调查的 9 个分离株形成了一个新的、支持良好的分支，而野生分离株与欧洲分离株聚集在一起。预测的蛋白结构分析显示，在分离株之间，α-和β-结构之间存在两个不同的区域。这是首次对哈萨克斯坦 raspberry 病毒的遗传组成进行了特征描述。