Laboratory of Fungal Interaction and Molecular Biology (donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba.
Faculty of Science, International College of Arts and Science, Yokohama City University.
Microbes Environ. 2020;35(3). doi: 10.1264/jsme2.ME20016.
Protists provide insights into the diversity and function of RNA viruses in marine systems. Among them, marine macroalgae are good targets for RNA virome analyses because they have a sufficient biomass in nature. However, RNA viruses in macroalgae have not yet been examined in detail, and only partial genome sequences have been reported for the majority of RNA viruses. Therefore, to obtain further insights into the distribution and diversity of RNA viruses associated with marine protists, we herein examined RNA viruses in macroalgae and a diatom. We report the putative complete genome sequences of six novel RNA viruses from two marine macroalgae and one diatom holobiont. Four viruses were not classified into established viral genera or families. Furthermore, a virus classified into Totiviridae showed a genome structure that has not yet been reported in this family. These results suggest that a number of distinct RNA viruses are widespread in a broad range of protists.
原生生物为我们深入了解海洋系统中 RNA 病毒的多样性和功能提供了线索。其中，海洋大型藻类是 RNA 病毒组分析的良好目标，因为它们在自然界中有足够的生物量。然而，海洋大型藻类中的 RNA 病毒尚未被详细研究，大多数 RNA 病毒只有部分基因组序列被报道。因此，为了更深入地了解与海洋原生生物相关的 RNA 病毒的分布和多样性，我们对大型藻类和一种硅藻中的 RNA 病毒进行了检测。我们报告了来自两种海洋大型藻类和一种硅藻共生体的 6 种新型 RNA 病毒的推定完整基因组序列。其中 4 种病毒未被归类为已建立的病毒属或科。此外，一种分类为Totiviridae 的病毒显示出该家族尚未报道的基因组结构。这些结果表明，许多不同的 RNA 病毒广泛存在于广泛的原生生物中。
