Department of Biological Science and Technology, Yonsei University, Wonju, Republic of Korea.
Biol Reprod. 2022 Jun 13;106(6):1098-1111. doi: 10.1093/biolre/ioac039.
Among the many calcium-binding proteins, S100A8, S100A9, and S100A12 play important roles in inflammation, innate immunity, and antimicrobial function, but their expression, regulation, and function at the maternal-conceptus interface in pigs are not fully understood. Therefore, we determined the expression and regulation of S100A8, S100A9, S100A12, and their receptor AGER at the maternal-conceptus interface in pigs. We found that S100A8, S100A9, and S100A12 mRNAs were expressed in the endometrium during the estrous cycle and pregnancy, with the greatest levels on Day (D) 12 of pregnancy, and AGER appeared at greater levels on D15 and D30 of pregnancy than on other days. The expression of S100A8, S100A9, and S100A12 was predominantly localized to epithelial cells in the endometrium, and they were detected in early-stage conceptus and later chorioallantoic tissues during pregnancy. AGER expression was localized to endometrial epithelial and stromal cells and chorionic epithelial cells. In endometrial explant tissues, the expression of S100A8, S100A9, and S100A12 was induced by estrogen, S100A8 by interleukin-1β, and AGER by interferon-γ. We further found that on D12 of pregnancy, the expression of S100A8, S100A9, and S100A12 decreased significantly in the endometria of gilts carrying conceptuses derived from somatic cell nuclear transfer. These results indicate that the expression of S100A8, S100A9, and S100A12 is dynamically regulated in response to conceptus-derived signals at the maternal-conceptus interface, suggesting that S100A8, S100A9, and S100A12 could play a critical role in regulating endometrial epithelial cell function and conceptus implantation to support the establishment and maintenance of pregnancy in pigs.
在众多钙结合蛋白中，S100A8、S100A9 和 S100A12 在后炎症、先天免疫和抗菌功能中发挥重要作用，但它们在猪的母体-胚胎界面的表达、调节和功能尚不完全清楚。因此，我们确定了猪母体-胚胎界面 S100A8、S100A9、S100A12 及其受体AGER 的表达和调节。我们发现 S100A8、S100A9 和 S100A12mRNA 在发情周期和妊娠期间在内膜中表达，妊娠第 12 天表达水平最高，AGER 在妊娠第 15 天和第 30 天的表达水平高于其他时间。S100A8、S100A9 和 S100A12 的表达主要定位于子宫内膜上皮细胞，在妊娠早期胚胎和晚期绒毛尿囊组织中均有检测到。AGER 表达定位于子宫内膜上皮细胞和基质细胞以及绒毛膜上皮细胞。在内膜组织培养物中，S100A8、S100A9 和 S100A12 的表达受雌激素诱导，S100A8 受白细胞介素-1β诱导，AGER 受干扰素-γ诱导。我们进一步发现，在妊娠第 12 天，核移植胚胎携带的母体子宫内膜中 S100A8、S100A9 和 S100A12 的表达显著降低。这些结果表明，S100A8、S100A9 和 S100A12 的表达在母体-胚胎界面受到胚胎衍生信号的动态调节，表明 S100A8、S100A9 和 S100A12 可能在调节子宫内膜上皮细胞功能和胚胎着床中发挥关键作用，以支持猪妊娠的建立和维持。
