Gmiter Dawid, Czerwonka Grzegorz, Drewnowska Justyna Malgorzata, Swiecicka Izabela, Kaca Wieslaw
Department of Microbiology, Jan Kochanowski University, 15 Swietokrzyska Street, 25-406, Kielce, Poland.
Departament of Microbiology, Institute of Biology, University of Bialystok, 1J Ciolkowskiego Street, 15-245, Bialystok, Poland.
Curr Microbiol. 2019 Feb;76(2):144-152. doi: 10.1007/s00284-018-1598-6. Epub 2018 Nov 17.
Proteus mirabilis is a pathogenic Gram-negative bacterium characterized by its ability to swarm across surfaces, which frequently leads to colonization of the urinary tract and causes severe infections. P. mirabilis strains are also well known from their self-recognition phenomenon, referred to as Dienes phenomenon. In this study, we present novel aspect of self-recognition, which is a hierarchy in terms of strains territoriality. We report the draft genome sequences of P. mirabilis K1609 and K670 strains exhibiting the strongest and the weakest territoriality, respectively. Our results indicated that K1609 is closely related to strain BB2000, a model system for self-recognition, comparing with the K670. We annotated genes associated with recognition of kin and swarming initiation control and indicated polymorphisms by which observed differences in territoriality might results from. The phenotypic and genomic features of both strains reveal their application as a model organisms for studying not only the mechanisms of kin-recognition but also strains territoriality, thus providing new approach to the phenomenon. Availability of these genome sequences may facilitate understanding of the interactions between P. mirabilis strains.
奇异变形杆菌是一种致病性革兰氏阴性菌，其特征在于能够在表面上群集移动，这常常导致其在泌尿道定植并引发严重感染。奇异变形杆菌菌株的自我识别现象（即迪内斯现象）也广为人知。在本研究中，我们展示了自我识别的一个新方面，即在菌株领地性方面存在等级制度。我们报告了奇异变形杆菌K1609和K670菌株的基因组草图序列，它们分别表现出最强和最弱的领地性。我们的结果表明，与K670相比，K1609与自我识别模型系统菌株BB2000密切相关。我们注释了与亲缘识别和群集起始控制相关的基因，并指出了观察到的领地性差异可能由此产生的多态性。这两种菌株的表型和基因组特征揭示了它们作为模型生物的应用价值，不仅可用于研究亲缘识别机制，还可用于研究菌株领地性，从而为该现象提供了新的研究方法。这些基因组序列的可得性可能有助于理解奇异变形杆菌菌株之间的相互作用。
