Genetics and Biotechnology Department, Strategic Center for Diabetes Research, College of medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Genetics Department, King Faisal Specialist Hospital and Research Center, Riyadh, Kingdom of Saudi Arabia.
Antimicrob Resist Infect Control. 2017 Nov 23;6:119. doi: 10.1186/s13756-017-0277-x. eCollection 2017.
is a common uropathogenic bacterium that can cause major complications in patients with long-standing indwelling catheters or patients with urinary tract anomalies. In addition, is a common cause of chronic osteomyelitis in Diabetic foot ulcer (DFU) patients. We isolated from a Diabetic ulcer patient. We examined levels of resistance against Nanosilver colloids, the commercial Nanosilver and silver containing bandages and commonly used antibiotics. We utilized next generation sequencing techniques (NGS), bioinformatics, phylogenetic analysis and pathogenomics in the characterization of the infectious pathogen.
was the first Nanosilver resistant isolate collected from a diabetic patient polyclonal infection. showed high levels of resistance against Nanosilver colloids, Nanosilver chitosan composite and the commercially available Nanosilver and silver bandages. The -SCDR1 genome size is 3,815,621 bp. with G + C content of 38.44%. -SCDR1 genome contains a total of 3533 genes, 3414 coding DNA sequence genes, 11, 10, 18 rRNAs (5S, 16S, and 23S), and 76 tRNAs. Our isolate contains all the required pathogenicity and virulence factors to establish a successful infection. SCDR1 isolate is a potential virulent pathogen that despite its original isolation site, the wound, can establish kidney infection and its associated complications. contains several mechanisms for antibiotics and metals resistance, including, biofilm formation, swarming mobility, efflux systems, and enzymatic detoxification.
is the first reported spontaneous Nanosilver resistant bacterial strain. possesses several mechanisms that may lead to the observed Nanosilver resistance.
是一种常见的尿路病原体，可导致长期留置导尿管或尿路畸形患者发生严重并发症。此外，也是糖尿病足溃疡（DFU）患者慢性骨髓炎的常见病因。我们从一位糖尿病溃疡患者中分离出了。我们检测了对纳米银胶体、市售纳米银和含银绷带以及常用抗生素的耐药水平。我们利用下一代测序技术（NGS）、生物信息学、系统发育分析和病原体组学对感染病原体进行了特征描述。
是首例从糖尿病患者多克隆感染中分离出的耐纳米银菌株。表现出对纳米银胶体、纳米银壳聚糖复合材料以及市售纳米银和含银绷带的高度耐药性。-SCDR1 基因组大小为 3815621bp，G+C 含量为 38.44%。-SCDR1 基因组共包含 3533 个基因，3414 个编码 DNA 序列基因，11 个、10 个、18 个 rRNA（5S、16S 和 23S）和 76 个 tRNA。我们的分离株包含建立成功感染所需的所有致病性和毒力因子。SCDR1 分离株是一种潜在的毒力病原体，尽管其最初的分离部位是伤口，但它可以建立肾脏感染及其相关并发症。含有几种抗生素和金属耐药机制，包括生物膜形成、群集运动、外排系统和酶解毒。
是首例报道的自发耐纳米银细菌株。具有几种可能导致观察到的纳米银耐药的机制。
