Wollen N, Kellett G L
Department of Biology, University of York.
Gut. 1988 Aug;29(8):1064-9. doi: 10.1136/gut.29.8.1064.
The regulation of the absorption and metabolism of glucose in rat small intestine by insulin was studied by the perfusion of isolated loops of proximal jejunum in vitro. The addition of an active, monomeric form of insulin, despentapeptide-insulin, to the serosal side of the intestine from normal rats inhibited luminal glucose absorption (421 (11) to 285 (11) mumol/h/g dry wt, p less than 0.001) and lactate production (340 (28) to 192 (26) mumol/h/g dry wt, p less than 0.001), but had no effect on glucose utilisation (231 (11) and 210 (16) mumol/h/g dry wt). The production of acute insulin deficiency by the injection of anti-insulin serum in vivo caused a marked inhibition of luminal glucose absorption (421 (11) to 240 (13) mumol/h/g dry wt, p less than 0.001), glucose utilisation (231 (11) to 48 (2) mumol/h/g dry wt, p less than 0.001) and lactate production (340 (28) to 94 (2) mumol/h/g dry wt, p less than 0.001) in vitro. The effects of insulin deficiency were reversed by despentapeptide-insulin in vitro, so that the rates of absorption and metabolism for intestine from insulin deficient and normal rats were similar in the presence of the modified insulin. All the effects caused by insulin deficiency and despentapeptide-insulin were apparent within minutes and could not be attributed to hyperglycaemia. It is concluded that rat small intestine is subject to rapid and direct regulation by insulin.
通过体外灌注大鼠空肠近端的孤立肠袢，研究了胰岛素对大鼠小肠葡萄糖吸收和代谢的调节作用。向正常大鼠小肠浆膜侧添加活性单体形式的胰岛素（去五肽胰岛素）可抑制肠腔葡萄糖吸收（从421（11）降至285（11）μmol/h/g干重，p<0.001）和乳酸生成（从340（28）降至192（26）μmol/h/g干重，p<0.001），但对葡萄糖利用无影响（分别为231（11）和210（16）μmol/h/g干重）。体内注射抗胰岛素血清导致急性胰岛素缺乏，可显著抑制体外肠腔葡萄糖吸收（从421（11）降至240（13）μmol/h/g干重，p<0.001）、葡萄糖利用（从231（11）降至48（2）μmol/h/g干重，p<0.001）和乳酸生成（从340（28）降至94（2）μmol/h/g干重，p<0.001）。体外去五肽胰岛素可逆转胰岛素缺乏的影响，因此在存在修饰胰岛素的情况下，胰岛素缺乏和正常大鼠小肠的吸收和代谢速率相似。胰岛素缺乏和去五肽胰岛素引起的所有效应在数分钟内即可显现，且不能归因于高血糖。结论是大鼠小肠受到胰岛素的快速直接调节。
