Kellett G L, Barker E D
Department of Biology, University of York, U.K.
Biochim Biophys Acta. 1989 Jul 21;992(1):128-30. doi: 10.1016/0304-4165(89)90059-7.
Rats were maintained on one of two standard commercial chow diets, Oxoid modified 41B or Bantin & Kingman rat and mouse diet, which differ in that linoleic acid comprises 27% and 44% of their total fatty acid content, respectively: the effects of bradykinin on the absorption, transmural transport and metabolism of glucose (5 mM) were then measured by the perfusion of isolated jejunal loops in vitro. With intestine from rats fed the Oxoid diet, bradykinin (100 nM in the serosal medium) caused significant increases in the rates of glucose absorption (34%, P less than 0.01) and lactate production (69%, P less than 0.01). These bradykinin-stimulated rates were the same, within experimental error, as those observed in the absence of bradykinin with intestine taken from rats fed the Bantin & Kingman diet and on which bradykinin had no effect. It is concluded that feeding rats with different commercial brands of apparently similar laboratory chow diets may result in significantly altered steady-states of glucose homeostasis in rat small intestine and in quite different sensitivities of glucose homeostasis to bradykinin. The possibility is considered that the differences in absorption might result in part from differences in the proportion of linoleic acid, which is known to enhance glucose absorption.
将大鼠饲养在两种标准商业普通饲料中的一种上，即Oxoid改良41B饲料或班廷与金曼大鼠和小鼠饲料，这两种饲料的区别在于亚油酸分别占其总脂肪酸含量的27%和44%：然后通过体外灌注分离的空肠袢来测量缓激肽对葡萄糖（5 mM）吸收、跨壁转运和代谢的影响。对于喂食Oxoid饲料的大鼠的肠道，缓激肽（浆膜培养基中为100 nM）导致葡萄糖吸收速率（34%，P<0.01）和乳酸生成速率（69%，P<0.01）显著增加。在实验误差范围内，这些缓激肽刺激的速率与取自喂食班廷与金曼饲料的大鼠的肠道在无缓激肽时观察到的速率相同，而缓激肽对其无影响。得出的结论是，用不同商业品牌的看似相似的实验室普通饲料喂养大鼠可能会导致大鼠小肠中葡萄糖稳态的稳定状态显著改变，以及葡萄糖稳态对缓激肽的敏感性截然不同。有人认为吸收差异可能部分源于亚油酸比例的差异，已知亚油酸可增强葡萄糖吸收。
