Yao Wangyuan, Zhang Hui, Jiang Xiong, Mehmood Khalid, Iqbal Mujahid, Li Aoyun, Zhang Jialu, Wang Yaping, Waqas Muhammad, Shen Yaoqin, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Hubei Three Gorges Polytechnic, Yichang, China.
Front Pharmacol. 2018 Nov 2;9:1251. doi: 10.3389/fphar.2018.01251. eCollection 2018.
Tibial dyschondroplasia (TD) is an abnormality of the growth cartilage that occurs in chickens and other rapidly growing avian species. This disease not only cause huge economic losses, but also greatly affects animal welfare. The total flavonoids of (TFRD) has been used to cure wide variety of diseases including bone fractures and osteoarthritis and osteoporosis. However, less information is available about the using of TFRD against the TD. The aim of this study was to determine the effect of TFRD on TD by regulating BMP-2 and Runx2 in chickens. A total of 200 birds were randomly divided into control, TD, TD recovery (TDR), and TFRD groups. All the groups were given standard diet with an addition of thiram (50 mg/kg) from days 3 to 7 in TD, TDR, and TFRD groups in order to induce TD in chickens. After the induction of TD, the birds of TFRD group were fed standard diet with the addition of TFRD at 20 mg/kg. Clinical results conveyed that TFRD can improve the growth performance of the TD chickens and recover normal activity, and it is more obvious than TDR. Gene expressions of BMP-2 and Runx2 were down-regulated during the development of the disease and were up-regulated obviously after TFRD treatment. In conclusion, TFRD not only decreased the mortality rate but also increased the growth performance of TD in chickens. In conclusion, TFRD plays important role in improving the growth performance, adjusting the relevant physiological indicators, and regulating BMP-2 and Runx2 in chickens.
胫骨软骨发育不良（TD）是一种发生在鸡和其他快速生长的禽类中的生长软骨异常疾病。这种疾病不仅会造成巨大的经济损失，还会极大地影响动物福利。[具体植物名称]总黄酮（TFRD）已被用于治疗包括骨折、骨关节炎和骨质疏松症在内的多种疾病。然而，关于TFRD用于治疗TD的信息较少。本研究的目的是通过调节鸡体内的骨形态发生蛋白-2（BMP-2）和Runx2来确定TFRD对TD的影响。总共200只鸡被随机分为对照组、TD组、TD恢复组（TDR）和TFRD组。在TD组、TDR组和TFRD组中，从第3天到第7天，所有组均给予添加了福美双（50毫克/千克）的标准日粮，以诱导鸡发生TD。在诱导出TD后，TFRD组的鸡被喂食添加了20毫克/千克TFRD的标准日粮。临床结果表明，TFRD可以改善TD鸡的生长性能并恢复正常活动，且比TDR组更明显。在疾病发展过程中，BMP-2和Runx2的基因表达下调，而在TFRD治疗后明显上调。总之，TFRD不仅降低了死亡率，还提高了TD鸡的生长性能。总之，TFRD在改善鸡的生长性能、调节相关生理指标以及调节BMP-2和Runx2方面发挥着重要作用。
