recA基因和蛋白质的序列。
Sequences of the recA gene and protein.
作者信息
Sancar A, Stachelek C, Konigsberg W, Rupp W D
出版信息
Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5. doi: 10.1073/pnas.77.5.2611.
Abstract
We have determined the nucleotide sequence of the recA gene of Escherichia coli; this permits the formulation of the primary structure for the recA protein. This structure is consistent with the amino acid composition of the tryptic peptides obtained from the recA protein. The coding region of the recA gene has 1059 base pairs, which specify 352 amino acids. The recA protein has alanine and phenylalanine as its NH2- and COOH-terminal amino acids, respectively, and has the following amino acid composition: Cys3 Asp20 Asn15 Met9 Thr17 Ser20 Glu30 Gln13 Pro10 Gly35 Ala38 Val22 Ile27 Leu31 Tyr7 Phe10 His2Lys27 Trp2 Arg14. Of the three cysteine residues, only two can be alkylated under reducing and denaturing conditions. The molecular weight of the recA polypeptide is 37,842.
摘要
我们已经测定了大肠杆菌recA基因的核苷酸序列；这使得我们能够构建recA蛋白的一级结构。该结构与从recA蛋白获得的胰蛋白酶肽段的氨基酸组成一致。recA基因的编码区有1059个碱基对，指定352个氨基酸。recA蛋白的NH2-末端和COOH-末端氨基酸分别为丙氨酸和苯丙氨酸，其氨基酸组成如下：半胱氨酸3、天冬氨酸20、天冬酰胺15、甲硫氨酸9、苏氨酸17、丝氨酸20、谷氨酸30、谷氨酰胺13、脯氨酸10、甘氨酸35、丙氨酸38、缬氨酸22、异亮氨酸27、亮氨酸31、酪氨酸7、苯丙氨酸10、组氨酸2、赖氨酸27、色氨酸2、精氨酸14。在三个半胱氨酸残基中，只有两个在还原和变性条件下可以被烷基化。recA多肽的分子量为37,842。
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