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Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis.

作者信息

Nohno T, Kasai Y, Saito T

机构信息

Department of Pharmacology, Kawasaki Medical School, Kurashiki, Japan.

出版信息

J Bacteriol. 1988 Sep;170(9):4097-102. doi: 10.1128/jb.170.9.4097-4102.1988.

DOI:10.1128/jb.170.9.4097-4102.1988
PMID:3045084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211414/
Abstract

The nucleotide sequence of a HinPI-HpaII restriction nuclease fragment which complemented a delta chlE strain of Escherichia coli was determined. Two open reading frames were deduced to be the structural genes for ChlE and ChlN proteins, which have molecular weights of 44,067 and 26,719, respectively. Both proteins were required for complementing a chromosomal deletion of the chlE locus. The chlE and chlN genes were transcribed from a common promoter, chlEp, located upstream of chlE. Transcriptional and translational signal sequences were recognized in this region.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05ad/211414/632660f3fa66/jbacter00187-0321-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05ad/211414/632660f3fa66/jbacter00187-0321-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05ad/211414/632660f3fa66/jbacter00187-0321-a.jpg

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J Bacteriol. 1982 Aug;151(2):788-99. doi: 10.1128/jb.151.2.788-799.1982.
2
Involvement of a gene of the chl E locus in the regulation of the nitrate reductase operon.
Mol Gen Genet. 1982;185(2):334-8. doi: 10.1007/BF00330808.
3
Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.硝酸还原酶和细胞色素b硝酸还原酶结构基因作为硝酸还原酶操纵子的组成部分。
J Mol Biol. 2013 Oct 9;425(19):3662-77. doi: 10.1016/j.jmb.2012.12.010. Epub 2012 Dec 28.
4
The genome sequence of Geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to Geobacter sulfurreducens.嗜金属还原地杆菌的基因组序列：与硫还原地杆菌相同和不同的代谢、生理及调控特征
BMC Microbiol. 2009 May 27;9:109. doi: 10.1186/1471-2180-9-109.
5
Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus.嗜热栖热菌钼辅因子生物合成蛋白C的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt 1):27-9. doi: 10.1107/S1744309106052560. Epub 2006 Dec 16.
6
Complex regulation of arsenite oxidation in Agrobacterium tumefaciens.根癌土壤杆菌中亚砷酸盐氧化的复杂调控
J Bacteriol. 2006 Feb;188(3):1081-8. doi: 10.1128/JB.188.3.1081-1088.2006.
7
ModE-dependent molybdate regulation of the molybdenum cofactor operon moa in Escherichia coli.大肠杆菌中钼辅因子操纵子moa的钼酸盐调控依赖于ModE。
J Bacteriol. 2000 Dec;182(24):7035-43. doi: 10.1128/JB.182.24.7035-7043.2000.
8
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Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10266-71. doi: 10.1073/pnas.97.18.10266.
9
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5
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Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
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J Biol Chem. 1984 May 10;259(9):5414-22.
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