Bauer Leonard, Antunović Maja, Ivanković Hrvoje, Ivanković Marica
Faculty of Chemical Engineering and Technology, University of Zagreb, Trg Marka Marulića 19, HR-10001 Zagreb, Croatia.
Biomimetics (Basel). 2024 Jan 4;9(1):30. doi: 10.3390/biomimetics9010030.
The occurrence of bone disorders is steadily increasing worldwide. Bone tissue engineering (BTE) has emerged as a promising alternative to conventional treatments of bone defects, developing bone scaffolds capable of promoting bone regeneration. In this research, biomimetic scaffolds based on ion-substituted calcium phosphates, derived from cuttlefish bone, were prepared using a hydrothermal method. To synthesize Mn-substituted scaffolds, three different manganese concentrations (corresponding to 1, 2.5, and 5 mol% Mn substitutions for Ca into hydroxyapatite) were used. Also, syntheses with the simultaneous addition of an equimolar amount (1 mol%) of two (Mg and Sr) or three ions (Mn, Mg, and Sr) were performed. A chemical, structural, and morphological characterization was carried out using X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. The effects of the ion substitutions on the lattice parameters, crystallite sizes, and fractions of the detected phases were discussed. Multi-substituted (Mn, Mg, and Sr) scaffolds were coated with polycaprolactone (PCL) using simple vacuum impregnation. The differentiation of human mesenchymal stem cells (hMSCs), cultured on the PCL-coated scaffold, was evaluated using histology, immunohistochemistry, and reverse transcription-quantitative polymerase chain reaction analyses. The expression of collagen I, alkaline phosphatase, and dentin matrix protein 1 was detected. The influence of PCL coating on hMSCs behavior is discussed.
全球范围内，骨疾病的发生率正在稳步上升。骨组织工程（BTE）已成为一种有前景的替代传统骨缺损治疗方法的技术，致力于开发能够促进骨再生的骨支架。在本研究中，采用水热法制备了基于来自乌贼骨的离子取代磷酸钙的仿生支架。为了合成锰取代的支架，使用了三种不同的锰浓度（分别对应于将1、2.5和5摩尔%的锰取代羟基磷灰石中的钙）。此外，还进行了同时添加等摩尔量（1摩尔%）的两种离子（镁和锶）或三种离子（锰、镁和锶）的合成。使用X射线衍射、傅里叶变换红外光谱和扫描电子显微镜进行了化学、结构和形态表征。讨论了离子取代对晶格参数、微晶尺寸和检测相分数的影响。采用简单的真空浸渍法在多取代（锰、镁和锶）支架上涂覆聚己内酯（PCL）。使用组织学、免疫组织化学和逆转录-定量聚合酶链反应分析评估在PCL涂层支架上培养的人间充质干细胞（hMSCs）的分化情况。检测了I型胶原蛋白、碱性磷酸酶和牙本质基质蛋白1的表达。讨论了PCL涂层对hMSCs行为的影响。
