Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, Helios #02-02, Singapore, 138667, Singapore.
Chem Commun (Camb). 2018 Dec 4;54(97):13641-13653. doi: 10.1039/c8cc08107g.
Small fluorescent molecules have been an important tool for fluorescence-based imaging, thanks to their technical simplicity, sensitivity and structural flexibility. However, probing specific intracellular systems with a small fluorescent molecule is not an easy task owing to the intricate nature of the cells. Ideal imaging probes should be highly permeable and be without background noise to allow undisrupted observations. These probes will unquestionably be more advantageous for interrogating intracellular architectures and dynamics without compromising the cellular integrity, and could therefore be applied for applications in the native cellular environment. This review will highlight the advances in design strategies for cell-permeable fluorescent probes through a diversity-oriented fluorescence library approach and rational design via computational-aided predictive models. Here, we discuss a series of cell-permeable probe applications for imaging specific cell types and intracellular biomolecules, as well as the cellular environment.
小分子荧光探针在基于荧光的成像中是一种重要的工具，这要归功于它们技术上的简单性、灵敏度和结构的灵活性。然而，由于细胞的复杂性，用小分子荧光探针探测特定的细胞内系统并不是一件容易的事。理想的成像探针应该具有高通透性和无背景噪声，以允许进行无干扰的观察。这些探针无疑将更有利于在不损害细胞完整性的情况下对细胞内结构和动力学进行探测，因此可应用于天然细胞环境中的应用。本文将通过多样性导向的荧光文库方法和基于计算辅助预测模型的合理设计，重点介绍用于设计可穿透细胞膜的荧光探针的设计策略的进展。在这里，我们讨论了一系列用于特定细胞类型和细胞内生物分子成像以及细胞环境的可穿透细胞膜的探针应用。
