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通过活细胞亲和基质和ABC CRISPRa文库将小鼠干细胞探针CDy1鉴定为ALDH2和Abcb1b。

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library.

作者信息

Miyamoto Naoki, Go Young-Hyun, Ciaramicoli Larissa Miasiro, Kwon Haw-Young, Kim Heon Seok, Bi Xuezhi, Yu Young Hyun, Kim Beomsue, Ha Hyung-Ho, Kang Nam-Young, Yun Seong-Wook, Kim Jin-Soo, Cha Hyuk-Jin, Chang Young-Tae

机构信息

Center for Self-assembly and Complexity, Institute for Basic Science (IBS) Pohang 37673 Republic of Korea

Department of Life Science, Sogang University 35 Baekbeom-ro Mapo-gu Seoul 04107 South Korea.

出版信息

RSC Chem Biol. 2021 Aug 20;2(6):1590-1593. doi: 10.1039/d1cb00147g. eCollection 2021 Dec 2.

DOI:10.1039/d1cb00147g
PMID:34977573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8637918/
Abstract

CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.

摘要

CDy1是用于重编程研究和再生医学中区分胚胎干细胞的强大工具。然而，CDy1的干细胞选择性机制尚未完全明确。在此，我们报告了通过活细胞亲和基质和ABC转运蛋白CRISPRa文库筛选所阐明的ALDH2和ABCB1作为CDy1的分子靶点。这两种独特的正交机制为特定细胞类型的多维度细胞区分提供了潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/bb30a398c5d5/d1cb00147g-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/1ab5cb767e41/d1cb00147g-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/5f7b47d08d34/d1cb00147g-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/93fb224df440/d1cb00147g-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/901007a2b20d/d1cb00147g-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/bb30a398c5d5/d1cb00147g-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/1ab5cb767e41/d1cb00147g-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/5f7b47d08d34/d1cb00147g-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/93fb224df440/d1cb00147g-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/901007a2b20d/d1cb00147g-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43ab/8637918/bb30a398c5d5/d1cb00147g-f5.jpg

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2
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Nat Commun. 2019 Mar 7;10(1):1111. doi: 10.1038/s41467-019-08990-9.
3
Advances in the design of cell-permeable fluorescent probes for applications in live cell imaging.
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Chem Commun (Camb). 2018 Dec 4;54(97):13641-13653. doi: 10.1039/c8cc08107g.
4
Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker.利用波形蛋白作为生物标志物，通过小分子荧光探针鉴定肿瘤起始细胞。
Angew Chem Int Ed Engl. 2018 Mar 5;57(11):2851-2854. doi: 10.1002/anie.201712920. Epub 2018 Feb 14.
5
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6
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