Miyamoto Naoki, Go Young-Hyun, Ciaramicoli Larissa Miasiro, Kwon Haw-Young, Kim Heon Seok, Bi Xuezhi, Yu Young Hyun, Kim Beomsue, Ha Hyung-Ho, Kang Nam-Young, Yun Seong-Wook, Kim Jin-Soo, Cha Hyuk-Jin, Chang Young-Tae
Center for Self-assembly and Complexity, Institute for Basic Science (IBS) Pohang 37673 Republic of Korea
Department of Life Science, Sogang University 35 Baekbeom-ro Mapo-gu Seoul 04107 South Korea.
RSC Chem Biol. 2021 Aug 20;2(6):1590-1593. doi: 10.1039/d1cb00147g. eCollection 2021 Dec 2.
CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.
CDy1是用于重编程研究和再生医学中区分胚胎干细胞的强大工具。然而，CDy1的干细胞选择性机制尚未完全明确。在此，我们报告了通过活细胞亲和基质和ABC转运蛋白CRISPRa文库筛选所阐明的ALDH2和ABCB1作为CDy1的分子靶点。这两种独特的正交机制为特定细胞类型的多维度细胞区分提供了潜力。
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