Aliramaji Farzad, Pourjam Ebrahim, Álvarez-Ortega Sergio, Afshar Farahnaz Jahanshahi, Pedram Majid
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Departamento de Biología y Geología, Física y Química Inorgánica, Universidad Rey Juan Carlos, Campus de Móstoles, 28933-Madrid, España.
J Nematol. 2018;50(3):437-452. doi: 10.21307/jofnem-2018-035.
One new and one known species of the genus Aphelenchoides from Iran are studied. Aphelenchoides giblindavisi n. sp. is mainly characterized by having five lines in the lateral fields at mid-body, and a single mucro with several tiny nodular protuberances, giving a warty appearance to it, as revealed by detailed scanning electron microscopic (SEM) studies. The new species is further characterized by having a body length of 546 to 795 μm in females and 523 to 679 μm in males, rounded lip region separated from the rest body by a shallow depression, 10 to 11 μm long stylet with small basal swellings, its conus shorter than the shaft ( m = 36-43), 52 to 69 µm long postvulval uterine sac (PUS), males with 16 to 18 μm long arcuate spicules, and three pairs of caudal papillae. The new species was morphologically compared with two species of the genus having five lines in the lateral fields namely A. paramonovi and A. shamimi and species having a warty-surfaced mucro at tail end and similar morphometric data ranges. The morphological features and morphometrics of the second studied species, A. helicus , agreed well with the data given for the type population. However, detailed study of fresh females revealed it has three drop-shaped stylet knobs and long PUS, making it typologically similar to the genus Robustodorus , meriting its taxonomic revision, i.e., transferring to it. In molecular phylogenetic analyses using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences, the new species formed a clade with A. heidelbergi in both SSU and LSU D2-D3 trees. The species A. helicus , however, clustered inside a well-supported clade of the genus Robustodorus in both trees, corroborating its newly proposed taxonomic placement as Robustodorus helicus n. comb.
对来自伊朗的滑刃属（Aphelenchoides）的一个新物种和一个已知物种进行了研究。吉布林达维滑刃线虫（Aphelenchoides giblindavisi）新种的主要特征是，在虫体中部的侧区有5条线，且尾尖有单个具几个微小瘤状突起的短尾，经详细扫描电子显微镜（SEM）研究显示，其外观呈疣状。该新物种的进一步特征为，雌虫体长546至795μm，雄虫体长523至679μm，圆形唇区通过浅凹陷与虫体其余部分分开，口针长10至11μm，基部有小肿胀，其圆锥部短于杆部（m = 36 - 43），阴门后子宫囊（PUS）长52至69μm，雄虫有长16至18μm的弓形交合刺，以及三对尾乳突。将该新物种与侧区有5条线的该属两个物种，即帕拉莫诺夫滑刃线虫（A. paramonovi）和沙米滑刃线虫（A. shamimi），以及尾端具疣状表面短尾且形态测量数据范围相似的物种进行了形态学比较。所研究的第二个物种，螺旋滑刃线虫（A. helicus），其形态特征和形态测量数据与模式种群给出的数据非常吻合。然而，对新鲜雌虫的详细研究表明，它有三个水滴形口针瘤和长的阴门后子宫囊，在类型学上使其与粗壮滑刃属（Robustodorus）相似，值得对其进行分类修订，即将其转移到该属。在使用部分小亚基和大亚基核糖体RNA基因（SSU和LSU rDNA）序列进行的分子系统发育分析中，该新物种在SSU和LSU D2 - D3树中均与海德堡滑刃线虫（A. heidelbergi）形成一个分支。然而，螺旋滑刃线虫物种在两棵树中均聚集在粗壮滑刃属一个得到充分支持的分支内，证实了其新提议的分类地位，即粗壮滑刃线虫（Robustodorus helicus）新组合。
