Hewlett T E, Dickson D W
J Nematol. 1993 Dec;25(4 Suppl):785-8.
Attachment of relatively low numbers of endospores from two isolates of Pasteuria spp. to several species of nematodes was consistently achieved in 2-5 minutes with a centrifugation technique. The rate of attachment of Pasteuria penetrans at 10 endospores/0.1 ml/tube to second-stage juveniles (J2) of Meloidogyne javanica, M. incognita race 1, M. incognita race 3, and M. arenaria races 1 and 2 in two tests averaged 4.4, 5.2, 0.1, 0.3, and 0 endospores per J2, respectively. The rate of attachment Pasteuria sp. at 10(3) endospores/0.1 ml/tube to individuals of Hoplolaimus galeatus, Belonolaimus longicaudatus, M. arenaria race 1, M. javanica, and M. incognita race 1 in two tests averaged 0.8, 0.04, 0, 0, and 0 endospores per nematode, respectively. The rate of attachment of P. penetrans to M. javanica at 10(3), 10, or 10 endospores/0.1 ml/tube from two tests averaged 1.0, 5.7, and 28.3 endospores per J2, respectively. All of the J2 had endospores attached following centrifugation in tubes with 10 and 10 endospores/0.1 ml/tube.
通过离心技术，在2至5分钟内始终能使来自巴斯德氏菌属两个分离株的相对少量内生孢子附着到几种线虫上。在两项试验中，穿刺巴斯德氏菌以每管10个内生孢子/0.1毫升的浓度附着到爪哇根结线虫、南方根结线虫1号小种、南方根结线虫3号小种以及花生根结线虫1号和2号小种的二期幼虫（J2）上的速率，平均分别为每个J2有4.4、5.2、0.1、0.3和0个内生孢子。在两项试验中，巴斯德氏菌属以每管10³个内生孢子/0.1毫升的浓度附着到强壮螺旋线虫、长针线虫、花生根结线虫1号小种、爪哇根结线虫和南方根结线虫1号小种个体上的速率，平均分别为每条线虫0.8、0.04、0、0和0个内生孢子。在两项试验中，穿刺巴斯德氏菌以每管10³、10或10个内生孢子/0.1毫升的浓度附着到爪哇根结线虫上的速率，平均分别为每个J2有1.0、5.7和28.3个内生孢子。在每管含10和10个内生孢子/0.1毫升的试管中离心后，所有J2都附着有内生孢子。
