Department of Chemistry, Chemistry Research Laboratory , University of Oxford , Mansfield Road , Oxford OX1 3TA , U.K.
Cancer Research U.K./MRC Oxford Institute for Radiation Oncology, Department of Oncology , University of Oxford , Old Road Campus Research Building , Oxford OX3 7DQ , U.K.
ACS Chem Biol. 2018 Dec 21;13(12):3354-3360. doi: 10.1021/acschembio.8b00858. Epub 2018 Dec 7.
Hypoxia, a condition of reduced oxygen, occurs in a wide variety of biological contexts, including solid tumors and bacterial biofilms, which are relevant to human health. Consequently, the development of chemical tools to study hypoxia is vital. Here we report a hypoxia-activated, small-molecule-mediated gene expression system using a bioreductive prodrug of the inducer isopropyl 1-thio-β-d-galactopyranoside. As a proof-of-concept we have placed the production of a green fluorescent protein under the control of hypoxia. Our system has the potential to be extended to regulate the production of any given protein of choice.
缺氧是一种氧气减少的情况，存在于广泛的生物环境中，包括实体肿瘤和与人类健康相关的细菌生物膜。因此，开发用于研究缺氧的化学工具至关重要。在这里，我们报告了一种使用诱导物异丙基 1-硫-β-d-半乳糖吡喃糖苷的生物还原前药的缺氧激活小分子介导的基因表达系统。作为概念验证，我们已经将绿色荧光蛋白的产生置于缺氧控制之下。我们的系统有可能扩展到调节任何所需蛋白质的产生。
