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普通变形杆菌的卵磷脂酶活性

Lecithinase activity of Proteus vulgaris.

作者信息

Postupa R, Postupová M

机构信息

District Hygienic Station, Hradec Králové, Czechoslovakia.

出版信息

J Hyg Epidemiol Microbiol Immunol. 1988;32(2):227-31.

PMID:3045200
Abstract

Lecithinase production is described as a new biochemical property of P. vulgaris strains grown in a selective agar medium containing brilliant green, crystal violet and lecithin (BCL agar), the authors' own modification of egg-yolk culture medium. By using this BCL agar as a medium inhibiting the swarming growth of P. vulgaris cultures the authors succeeded in identifying 12 lecithinase-positive strains among the P. vulgaris isolates obtained from patients with Crohn's disease. Of 50 P. mirabilis strains tested in parallel none gave the positive test for lecithinase production in this medium.

摘要

卵磷脂酶的产生被描述为在含有亮绿、结晶紫和卵磷脂的选择性琼脂培养基(BCL琼脂)中生长的普通变形杆菌菌株的一种新的生化特性,这是作者对蛋黄培养基的自行改良。通过使用这种BCL琼脂作为抑制普通变形杆菌培养物群游生长的培养基,作者成功地从克罗恩病患者分离出的普通变形杆菌中鉴定出12株卵磷脂酶阳性菌株。在同时检测的50株奇异变形杆菌菌株中,没有一株在这种培养基中产生卵磷脂酶呈阳性反应。

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